Fis binding to the recombinational enhancer of the Hin DNA inversion system.
Fis binding to the recombinational enhancer of the Hin DNA inversion system.
复制标题
Fis 与 Hin DNA 倒转系统的重组增强子结合。
DOI：
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发表时间：
1987
影响因子：
10.5
通讯作者：
Melvin I. Simon
中科院分区：
文献类型：
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作者：
M. Bruist;A. C. Glasgow;Reid C. Johnson;Melvin I. Simon
沙门氏菌中Hin倒位系统的重组增强子在大肠杆菌宿主因子Fis存在下刺激体外重组150倍。为了了解增强子和Fis在刺激Hin介导的倒置反应中的作用，我们使用了核酸酶和化学保护/干扰研究以及凝胶阻滞试验来研究Fis和重组增强子之间的相互作用。这些研究结合突变分析确定了Fis结合和功能所需的增强子序列。Fis以不同的亲和力与增强子序列内的两个结构域结合。Fis在每个结构域的结合独立于其他结构域的占用，并且似乎是DNA螺旋的相对面。这些结果支持了重组增强子在Hin介导的倒位中的作用的模型，其中结合在重组位点处的Hin和结合到重组增强子的每个结构域的Fis之间的相互作用导致具有适当对齐和拓扑结构的结构以促进DNA倒位。
The recombinational enhancer of the Hin inversion system in Salmonella stimulates recombination in vitro 150-fold in the presence of the Escherichia coli host factor Fis. To gain an understanding of the roles of the enhancer and Fis in stimulating the Hin-mediated inversion reaction, we have used nuclease and chemical protection/interference studies and gel retardation assays to examine the interactions between Fis and the recombinational enhancer. These studies combined with mutational analysis defined the enhancer sequences required for Fis binding and function. Fis binds with different affinities to two domains within the enhancer sequence. The binding of Fis at each domain is independent of the occupancy of the other domain and appears to be to opposite faces of the DNA helix. These results support a model for the role of the recombinational enhancer in Hin-mediated inversion in which the interaction between Hin bound at recombination sites and Fis bound to each domain of the recombinational enhancer results in a structure with the proper alignment and topology to promote DNA inversion.
DOI：
10.1101/sqb.1984.049.01.045
发表时间：
1984-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
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作者：
COZZARELLI, NR;KRASNOW, MA;WHITE, JH
通讯作者：
WHITE, JH