Discover of a Novel SNP Highly Associated with Chicken Blue Egg

Discover of a Novel SNP Highly Associated with Chicken Blue Egg
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发现与蓝鸡蛋高度相关的新 SNP

DOI:
10.3923/javaa.2011.92.95
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发表时间:
2011
影响因子:
--
通讯作者:
Deng, X. M.
Deng, X. M.
中科院分区:
--
文献类型:
--
作者:
Wang, Z. P.;Wang, X. T.;Liu, R. F.;Wang, A. R.;Li, J. Y.;Deng, X. M.

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蓝蛋是由位于鸡1号染色体短臂上的一个显性突变Ocyan(O)引起的,但其确切的遗传机制尚不清楚。在这项研究中,我们使用126只褐壳鸡和202只蓝壳鸡发现了一个SNP(g.67419892- 67419904 del 13)与蓝蛋显着相关。蓝壳鸡中g.67419892- 67419904 del 13的等位基因频率和基因型频率(92.5%和87.1%)显著高于褐壳鸡(10.7%和8.7%,p < 0.0001)。利用149个蓝壳雄鱼和褐壳雌鱼交配的F(2)后代,估计O和g.67419892- 67419904 del 13之间的遗传距离超过2cM(LOD = 13.51)。该标记可用于鸡O基因的克隆和鸡品种的标记辅助选择。
Blue egg is caused by a dominant mutation, Oocyan (O) which is previous located on short arm of chicken chromosome 1 but precise genetic mechanism underlying blue egg is still unknown so far. In this study, we found a SNP (g.67419892-67419904del13) significantly associated with blue egg using 126 browned-shelled chickens and 202 blue-shelled chickens. The allelic and genotypic frequencies of g.67419892-67419904del13 are significantly higher in blue-shelled chickens (92.5 and 87.1%) compared with brown-shelled chickens (10.7 and 8.7%, p < 0.0001). Moreover, the genetic distance between O and g.67419892-67419904del13 is estimated to be over 2 cM (LOD = 13.51) using 149 F(2) offspring from mating between blue-shelled males and brown-shelled females. Apparently, the marker is useful for the cloning of O and marker aid selection of blue-shelled chicken breed.
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