A medium-throughput crystallization approach.
A medium-throughput crystallization approach.
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中等通量的结晶方法。
DOI：
10.1107/s0907444902013938
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
C. Cambillau
中科院分区：
文献类型：
--
作者：
G. Sulzenbacher;A. Gruez;V. Roig;S. Spinelli;Christel Valencia;F. Pagot;R. Vincentelli;C. Bignon;Aurélia Salomoni;S. Grisel;Damien Maurin;C. Huyghe;Kent Johansson;A. Grassick;A. Roussel;Y. Bourne;S. Perrier;L. Miallau;P. Cantau;E. Blanc;M. Genevois;Alain Grossi;A. Zenatti;V. Campanacci;C. Cambillau
中等规模结构基因组学计划的第一个结果清楚地证明了使用基于两步程序的中等通量结晶方法的价值：采用机器人的大型筛选步骤，然后手动或自动优化结晶条件。结构基因组学程序基于Gateway载体pDEST 17中的克隆，在N-末端引入长的21个残基尾。到目前为止，这条尾巴似乎还没有阻碍结晶。在10个月内，25种蛋白质进行了结晶; 13种产生了晶体，其中10种产生了可用的数据集，5种产生了结构。此外，使用机器人分配50-200 nl液滴的结果表明，较小的蛋白质样品可用于结晶。这些仍然是部分的结果可能会表明现在和未来的方向，为那些谁必须作出关键的选择，他们的结晶平台在结构基因组学计划。
The first results of a medium-scale structural genomics program clearly demonstrate the value of using a medium-throughput crystallization approach based on a two-step procedure: a large screening step employing robotics, followed by manual or automated optimization of the crystallization conditions. The structural genomics program was based on cloning in the Gateway vectors pDEST17, introducing a long 21-residue tail at the N-terminus. So far, this tail has not appeared to hamper crystallization. In ten months, 25 proteins were subjected to crystallization; 13 yielded crystals, of which ten led to usable data sets and five to structures. Furthermore, the results using a robot dispensing 50-200 nl drops indicate that smaller protein samples can be used for crystallization. These still partial results might indicate present and future directions for those who have to make crucial choices concerning their crystallization platform in structural genomics programs.
影响因子：
56.9
作者：
HENDRICKSON, WA
通讯作者：
HENDRICKSON, WA