Plasma Proteomics Characteristics of Subclinical Vitamin E Deficiency of Dairy Cows During Early Lactation.
Plasma Proteomics Characteristics of Subclinical Vitamin E Deficiency of Dairy Cows During Early Lactation.
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泌乳早期奶牛亚临床维生素E缺乏症的血浆蛋白质组学特征
DOI：
10.3389/fvets.2021.723898
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发表时间：
2021
影响因子：
3.2
通讯作者：
Xia C
中科院分区：
文献类型：
--
作者：
Qian W;Yu H;Zhang C;Zhang H;Fu S;Xia C
维生素E（VE）是奶牛必需的脂溶性营养素。维生素 E 缺乏会导致免疫抑制和氧化应激，并增加奶牛产后早期生殖障碍的易感性。但目前关于VE缺乏的血浆蛋白质组学研究尚未见报道。因此，本研究的目的是了解亚临床VE缺乏症奶牛产后早期血液蛋白谱的变化。在本研究中，通过串联质量标签 (TMT) 分析了 14 头健康奶牛（>4 μg/ml α-生育酚）和 13 头亚临床 VE 缺陷奶牛（2–3 μg/ml α-生育酚）的血浆蛋白水平。结果显示，与健康对照相比，亚临床VE缺乏症奶牛血浆中存在26个差异表达蛋白（DEP）。 21种蛋白质下调，5种上调，其中蛋白质-蛋白质相互作用（PPI）网络中的8种蛋白质存在直接相互作用。这些蛋白主要参与MAPK信号通路、泛酸和辅酶A（CoA）生物合成、PPAR信号通路和糖基磷脂酰肌醇（GPI）锚定生物合成。通过文献查阅，发现PPI中排名前4位的DEP（APOC3、APOC4、SAA4、PHLD）和1个重要蛋白（VNN1），并通过ELISA和Western blot进一步验证。 ELISA检测显示APOC3、VNN1、SAA4的表达水平显着低于健康对照。通过蛋白质印迹检测，VNN1 显着低于健康对照。 VNN1与奶牛亚临床VE缺乏密切相关，可以作为潜在的生物标志物。为进一步研究VE缺乏病理机制和抗氧化应激奠定基础。
Vitamin E (VE) is an essential fat-soluble nutrient for dairy cows. Vitamin E deficiency leads to immune suppression and oxidative stress and increases the susceptibility of cows to reproductive disorders in the early post-partum period. However, studies on plasma proteomics of VE deficiency have not been reported so far. Therefore, the purpose of this study was to understand the changes of blood protein profile in cows with subclinical VE deficiency in the early post-partum period. In this study, plasma protein levels of 14 healthy cows (>4 μg/ml α-tocopherol) and 13 subclinical VE-deficient cows (2–3 μg/ml α-tocopherol) were analyzed by tandem mass tag (TMT). The results showed that there were 26 differentially expressed proteins (DEPs) in the plasma of cows with subclinical VE deficiency compared with healthy controls. Twenty-one kinds of proteins were downregulated, and five kinds were upregulated, among which eight proteins in protein–protein interactions (PPI) network had direct interaction. These proteins are mainly involved in the MAPK signaling pathway, pantothenic acid and coenzyme A (CoA) biosynthesis, PPAR signaling pathway, and glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The top four DEPs in PPI (APOC3, APOC4, SAA4, PHLD) and one important protein (VNN1) by literature review were further verified by ELISA and Western blot. The expression levels of APOC3, VNN1, and SAA4 were significantly lower than those of healthy controls by ELISA. VNN1 was significantly lower than those of healthy controls by Western blot. VNN1 is closely related to dairy cow subclinical VE deficiency and can be a potential biomarker. It lays a foundation for further research on the lack of pathological mechanism and antioxidative stress of VE.
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影响因子：
1.7
作者：
Folnozic, I.;Turk, R.;Samardzija, M.
通讯作者：
Samardzija, M.
影响因子：
5
作者：
Bukowska, Alicja;Zacharias, Ines;Goette, Andreas
通讯作者：
Goette, Andreas
影响因子：
5.3
作者：
Berruyer, C;Martin, FM;Galland, F
通讯作者：
Galland, F
影响因子：
8.8
作者：
Joy, Minju;Chakraborty, Kajal
通讯作者：
Chakraborty, Kajal
DOI：
10.1006/bbrc.1997.6475
发表时间：
1997-04-17
影响因子：
3.1
作者：
Jones, DR;Avila, MA;VarelaNieto, I
通讯作者：
VarelaNieto, I