Glucose metabolism and recycling by hepatocytes of OB/OB and ob/ob mice.
Glucose metabolism and recycling by hepatocytes of OB/OB and ob/ob mice.
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OB/OB 和 ob/ob 小鼠肝细胞的葡萄糖代谢和回收。
DOI：
10.1152/ajpendo.1990.259.3.e389
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Katz,J
中科院分区：
文献类型：
--
作者：
Lahtela,JT;Wals,PA;Katz,J
从禁食24 h的ob/ob（肥胖糖尿病）小鼠及其瘦型（OB/OB）同胞的肝脏制备肝细胞。将肝细胞与浓度为20至120 mM的[U-14 C，2- 3 H]-、[U-14 C，3- 3 H]-和[U-14 C，6- 3 H]葡萄糖孵育。14 C主要以CO2、糖原和乳酸盐形式回收。氚在水和糖原中回收。[2- 3 H]葡萄糖标记产物的产率为[U-14 C]葡萄糖的2 - 3倍。[3- 3 H]-和[6- 3 H]-葡萄糖的产率相似，是[U-14 C]-葡萄糖的1.3-1.7倍。在40 mM时，肥胖小鼠对葡萄糖的总利用率约为瘦小鼠的两倍，但在120 mM时几乎没有差异。计算葡萄糖和葡萄糖6-磷酸之间的再循环速率。根据糖原中2- 3 H/U-14 C比率计算葡萄糖再循环速率的公式见附录。我们的研究结果表明，1）肥胖糖尿病小鼠肝细胞对葡萄糖的利用超过了其瘦对照组，2）两组中葡萄糖磷酸化的速率大大超过葡萄糖摄取和糖原合成的速率，3）葡萄糖磷酸化代表了高葡萄糖激酶速率和葡萄糖6-磷酸水解之间的差异，和4）葡萄糖碳在葡萄糖6-磷酸和丙酮酸之间的再循环发生在小鼠肝细胞内。
Hepatocytes were prepared from livers of ob/ob (obese diabetic) mice and their lean (OB/OB) siblings that had been fasted for 24 h. The hepatocytes were incubated with [U-14C, 2-3H]-, [U-14C, 3-3H]-, and [U-14C, 6-3H]glucose at concentrations from 20 to 120 mM. 14C was recovered mainly in CO2, glycogen, and lactate. Tritium was recovered in water and glycogen. The yield in labeled products from [2-3H]glucose ranged from two to three times that from [U-14C]glucose. The yields from [3-3H]- and [6-3H]glucose were similar, and 1.3-1.7 times that from [U-14C]glucose. At 40 mM, total utilization of glucose by obese mice was about twice that for lean mice, but there was little difference at 120 mM. The rate of recycling between glucose and glucose 6-phosphate was calculated. An equation to calculate the rate of recycling of glucose from the 2-3H/U-14C ratio in glycogen is derived in the APPENDIX. Our results show that 1) the utilization of glucose by hepatocytes from obese diabetic mice exceeds that of their lean controls, 2) the rate of glucose phosphorylation in both groups greatly exceeds glucose uptake and the rate of glycogen synthesis, 3) glucose phosphorylation represents a difference between a high glucokinase rate and hydrolysis of glucose 6-phosphate, and 4) recycling of glucose carbon between glucose 6-phosphate and pyruvate occurs within mouse hepatocytes.
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胰高血糖素和胰岛素对正常和遗传性肥胖 (ob/ob) 小鼠灌注肝脏中脂肪酸合成和糖原降解的影响。
影响因子：
4.1
作者：
G. Y. Ma;C. Gove;D. Hems
通讯作者：
D. Hems
正常和遗传性肥胖高血糖（ob-ob）小鼠离体灌注肝脏的碳水化合物代谢。
DOI：
10.1042/bj1250773
发表时间：
1971
期刊：
The Biochemical journal
影响因子：
--
作者：
J. Elliott;D. Hems;A. Beloff
通讯作者：
A. Beloff
DOI：
10.1042/bj1800389
发表时间：
1979-05
期刊：
The Biochemical journal
影响因子：
--
作者：
J. Katz;S. Golden;P. Wals
通讯作者：
J. Katz;S. Golden;P. Wals
肥胖高血糖小鼠肝脏的适应性酶反应
DOI：
10.1016/0020-711x(75)90020-8
发表时间：
1975
期刊：
International Journal of Biochemistry
影响因子：
--
作者：
M. Kaplan;G. Fried
通讯作者：
G. Fried
无论是否肥胖，轻度 II 型糖尿病都会显着增加吸收后状态和葡萄糖输注期间的葡萄糖循环。
影响因子：
15.9
作者：
Suad Efendić;S. Karlander;Mladen Vranic
通讯作者：
Mladen Vranic