Unorthodox PCNA Binding by Chromatin Assembly Factor 1.
Unorthodox PCNA Binding by Chromatin Assembly Factor 1.
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DOI：
10.3390/ijms231911099
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发表时间：
2022-09-21
影响因子：
5.6
通讯作者：
Verreault, Alain
中科院分区：
文献类型：
--
作者：
Nair, Amogh Gopinathan;Rabas, Nick;Lejon, Sara;Homiski, Caleb;Osborne, Michael J.;Cyr, Normand;Sverzhinsky, Aleksandr;Melendy, Thomas;Pascal, John M.;Laue, Ernest D.;Borden, Katherine L. B.;Omichinski, James G.;Verreault, Alain
真核生物DNA复制叉是一系列酶的中枢，这些酶持续地合成DNA，传播DNA甲基化和其他表观遗传标记，进行质量控制，修复新生DNA，并将这些DNA包装成染色质。许多参与这些时空相关过程的酶通过与增殖细胞核抗原（PCNA）结合来发挥其功能。一个长期存在的问题是，过多的rna结合酶是如何发挥它们的活性而不相互干扰的。作为破解这一复杂调控的第一步，我们研究了染色质组装因子1 （caf1）如何与PCNA结合。我们证明了caf1以一种迄今为止尚未表征的方式与PCNA结合，这种方式依赖于阳离子- π (π)相互作用。精氨酸残基在ca -1同源物中保守，但在其他PCNA结合蛋白中不存在，它插入通常由含有典型PCNA相互作用肽（PIPs）的蛋白占据的疏水口袋中。这种精氨酸的突变破坏了ca -1结合PCNA和组装染色质的能力。caf1 p150亚基的PIP位于明显的长α-螺旋（119个氨基酸）的末端c端，该螺旋已被报道与DNA结合。螺旋的长度和c端PIP的存在在从酵母到人类的许多物种中都是进化保守的。这种在pip中终止的很长的DNA结合线圈的排列可能有助于协调DNA和PCNA通过ca -1的结合。
The eukaryotic DNA replication fork is a hub of enzymes that continuously act to synthesize DNA, propagate DNA methylation and other epigenetic marks, perform quality control, repair nascent DNA, and package this DNA into chromatin. Many of the enzymes involved in these spatiotemporally correlated processes perform their functions by binding to proliferating cell nuclear antigen (PCNA). A long-standing question has been how the plethora of PCNA-binding enzymes exert their activities without interfering with each other. As a first step towards deciphering this complex regulation, we studied how Chromatin Assembly Factor 1 (CAF-1) binds to PCNA. We demonstrate that CAF-1 binds to PCNA in a heretofore uncharacterized manner that depends upon a cation-pi (π) interaction. An arginine residue, conserved among CAF-1 homologs but absent from other PCNA-binding proteins, inserts into the hydrophobic pocket normally occupied by proteins that contain canonical PCNA interaction peptides (PIPs). Mutation of this arginine disrupts the ability of CAF-1 to bind PCNA and to assemble chromatin. The PIP of the CAF-1 p150 subunit resides at the extreme C-terminus of an apparent long α-helix (119 amino acids) that has been reported to bind DNA. The length of that helix and the presence of a PIP at the C-terminus are evolutionarily conserved among numerous species, ranging from yeast to humans. This arrangement of a very long DNA-binding coiled-coil that terminates in PIPs may serve to coordinate DNA and PCNA binding by CAF-1.
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影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
DOI：
10.1016/bs.enz.2016.03.003
发表时间：
2016
期刊：
The Enzymes
影响因子：
--
作者：
Boehm EM;Gildenberg MS;Washington MT
通讯作者：
Washington MT
影响因子：
2.1
作者：
Iida, T;Suetake, I;Tsurimoto, T
通讯作者：
Tsurimoto, T
DOI：
10.1073/pnas.0406540102
发表时间：
2005-02-08
影响因子：
11.1
作者：
Kontopidis, G;Wu, SY;Walkinshaw, MD
通讯作者：
Walkinshaw, MD
影响因子：
64.5
作者：
KRISHNA, TSR;KONG, XP;KURIYAN, J
通讯作者：
KURIYAN, J