Up-Regulation of HSFA2c and HSPs by ABA Contributing to Improved Heat Tolerance in Tall Fescue and Arabidopsis.
Up-Regulation of HSFA2c and HSPs by ABA Contributing to Improved Heat Tolerance in Tall Fescue and Arabidopsis.
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ABA 上调 HSFA2c 和 HSPs 有助于提高高羊茅和拟南芥的耐热性
DOI：
10.3390/ijms18091981
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发表时间：
2017-09-15
影响因子：
5.6
通讯作者：
Huang B
中科院分区：
文献类型：
--
作者：
Wang X;Zhuang L;Shi Y;Huang B
已知脱落酸（阿坝）在调节植物对各种非生物胁迫的耐受性中发挥作用，但阿坝对耐热性的影响是否与其对热胁迫转录因子（HSFs）和热激蛋白（HSPs）的调节有关尚不清楚。本研究的目的是确定是否可以提高高羊茅（Festuca arundinacea Schreb.）的耐热性。阿坝对HSFs和HSPs的调节作用。高羊茅ABA应答转录因子FaAREB 3和FaDREB 2A能够与高羊茅热应激转录因子A2 c（FaHSFA 2c）启动子的顺式元件结合。外源阿坝（5 μM）处理提高了高羊茅的耐热性，表现为提高了高温胁迫（37/32 °C，白天/夜晚）下叶片的光化学效率和膜稳定性。在阿坝处理的植物中，FaHSFA 2c、几种高羊茅热休克蛋白（FaHSPs）和ABA响应性转录因子的表达水平上调。拟南芥热应激转录因子A2（AtHSFA 2）的缺陷抑制ABA诱导AtHSPs的表达，ABA提高了拟南芥的耐热性。这些结果表明，HSFA 2在ABA介导的植物耐热性中起重要作用，FaAREB 3和FaDREB 2A可能作为上游反式作用因子，调控FaHSFA 2c和下游FaHSPs的转录活性，从而提高植物的耐热性。
Abscisic acid (ABA) is known to play roles in regulating plant tolerance to various abiotic stresses, but whether ABA’s effects on heat tolerance are associated with its regulation of heat stress transcription factors (HSFs) and heat shock proteins (HSPs) is not well documented. The objective of this study was to determine whether improved heat tolerance of tall fescue (Festuca arundinacea Schreb.) by ABA was through the regulation of HSFs and HSPs. ABA-responsive transcriptional factors, ABA-responsive element binding protein 3 (FaAREB3) and dehydration-responsive element binding protein 2A (FaDREB2A) of tall fescue, were able to bind to the cis-elements in the promoter of tall fescue heat stress transcription factor A2c (FaHSFA2c). Exogenous ABA (5 μM) application enhanced heat tolerance of tall fescue, as manifested by increased leaf photochemical efficiency and membrane stability under heat stress (37/32 °C, day/night). The expression levels of FaHSFA2c, several tall fescue HSPs (FaHSPs), and ABA-responsive transcriptional factors were up-regulated in plants treated with ABA. Deficiency of Arabidopsis heat stress transcription factor A2 (AtHSFA2) suppressed ABA-induction of AtHSPs expression and ABA-improved heat tolerance in Arabidopsis. These results suggested that HSFA2 plays an important role in ABA-mediated plant heat tolerance, and FaAREB3 and FaDREB2A may function as upstream trans-acting factors and regulate transcriptional activity of FaHSFA2c and the downstream FaHSPs, leading to improved heat tolerance.
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影响因子：
7.4
作者：
Haberer, G;Hindemitt, T;Mayer, KFX
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