Chromosomal disorders and nuclear and cell destruction in cleaving human embryos.
Chromosomal disorders and nuclear and cell destruction in cleaving human embryos.
复制标题
人类胚胎分裂过程中的染色体紊乱以及细胞核和细胞破坏。
DOI：
10.1387/ijdb.041909sd
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
D. Toncheva
中科院分区：
文献类型：
--
作者：
S. Delimitreva;R. Zhivkova;I. Vatev;D. Toncheva
三种类型的植入前胚胎发育缺陷有助于分裂人类胚胎的发育停滞：卵裂球碎裂，异常核状态和染色体异常。关于人类受精卵第一次有丝分裂周期中这些异常的关系和连续性的数据是有争议的，目前主要是经验性的。在这项研究中，我们进行了同时评估的卵裂球碎裂，核凋亡的变化和四个染色体（1，5，19和X或18，21，X和Y）在193人胚胎的倍性。另一组28个胚胎进行TUNEL以确认卵裂球核中的凋亡。在形态良好的胚胎中，近1/10的卵裂球可见凋亡染色质核，而在碎裂率大于20%的胚胎中，超过1/5的卵裂球可见凋亡染色质核。在85.2%成功测试的胚胎中登记了正确的染色体数目。染色质凋亡变化是染色体FISH检测成功的唯一限制因素。近1/2的胚胎至少有一个凋亡细胞核染色体异常。对于仅含有正常细胞核的胚胎，倍体正常率大于89%。在细胞破碎程度显著的胚胎中，整倍体率较高（66%）。中度细胞碎裂与染色质显著增加和染色体疾病无关。在相当一部分异常卵裂球中，染色质损伤先于细胞碎裂。人类卵裂球的核破坏通过染色质损伤的不同阶段的荧光照片来说明。
Three types of defects of preimplantation embryogenesis contribute to the developmental arrest of cleaving human embryos: blastomere fragmentation, abnormal nuclear status and chromosomal disorders. Data concerning the relation and succession of these abnormalities during first mitotic cycles of the human zygote are controversial and mainly empirical at present. In this study we have performed simultaneous evaluation of blastomere fragmentation, nuclear apoptotic changes and the ploidy of four chromosomes (1, 5, 19 and X or 18, 21, X and Y) in 193 human embryos. Another group of 28 embryos was subjected to TUNEL for confirmation of apoptosis in blastomere nuclei. Nuclei with apoptotic chromatin were seen in nearly 1/10 of blastomeres of embryos with good morphology and in more than 1/5 of blastomeres of embryos with more than 20% fragmentation. The correct number of investigated chromosomes was registered in 85.2% of successfully tested embryos. Chromatin apoptotic changes are the only limiting factor for the success of chromosomal FISH tests. Nearly 1/2 of embryos with at least one apoptotic nucleus were chromosomally abnormal. For the embryos that contain only normal nuclei, the rate of ploid normality was more than 89%. The rate of euploidy was higher (66%) in embryos with a significant degree of cell fragmentation. Moderate cell fragmentation was not related to significant increase of chromatin and chromosomal disorders. In a substantial portion of abnormal blastomeres, chromatin damage preceded cell fragmentation. Nuclear destruction in human blastomeres was illustrated by fluorograms of different stages of chromatin lesions.
卵子和胚胎存活的遗传调控。
DOI：
10.1093/humrep/13.suppl_3.178
发表时间：
1998
期刊：
Human reproduction (Oxford, England)
影响因子：
--
作者：
Warner,CM;Cao,W;Exley,GE;McElhinny,AS;Alikani,M;Cohen,J;Scott,RT;Brenner,CA
通讯作者：
Brenner,CA
影响因子：
6.1
作者：
Antczak, M;Van Blerkom, J
通讯作者：
Van Blerkom, J