A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk
A self-assembling RNA aptamer-based nanoparticle sensor for fluorometric detection of Neomycin B in milk
复制标题
基于自组装 RNA 适体的纳米颗粒传感器用于牛奶中新霉素 B 的荧光检测
DOI：
10.1007/s00216-016-9441-z
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发表时间：
2016-03
影响因子：
4.3
通讯作者：
Li Fu-Rong
中科院分区：
文献类型：
--
作者：
Ling Kai;Jiang Hongyan;Zhang Linlin;Li Yang;Yang Lu;Qiu Chen;Li Fu-Rong
迄今为止，关于基于RNA适体的小分子检测生物传感器的开发报道很少。其原因可能是RNA在生物环境中对核酸酶的抵抗力较弱。在这项研究中，我们开发了一种基于RNA适配体的金纳米颗粒（AuNP）传感器，用于荧光检测牛奶中的新霉素B。该适体传感器依赖于RNA适体/新霉素B复合物的自组装和AuNPs的荧光猝灭。该传感器的检测限为0.01 μM，在牛奶中的线性动态范围为0.1 ~ 10 μM，对新霉素b具有良好的选择性。由于RNA修饰的AuNPs具有较短的RNA片段和核酸酶抑制能力，因此在实验过程中RNA序列保持稳定。这项工作将作为开发基于RNA适体的新型生物传感器的一个例子。摘要基于RNA适配体的纳米颗粒传感器具有较高的结合亲和力和选择性，可用于牛奶中新霉素B的检测。这种适配体传感器依赖于适配体/配体复合物的自组装和金纳米颗粒（AuNPs）的荧光猝灭。由于RNA修饰的AuNPs具有较短的RNA片段和核酸酶抑制能力，因此RNA序列在检测过程中保持稳定。
To date, there are few reports regarding the development of RNA aptamer-based biosensors for the detection of small molecules. The possible reason is attributed to the weak nuclease resistance of RNA in biological environments. In this study, we have developed an RNA aptamer-based gold nanoparticle (AuNP) sensor for fluorometric detection of Neomycin B in milk. This aptasensor depends on the self-assembly of the RNA aptamer/Neomycin B complex and fluorescence quenching by AuNPs. This biosensor exhibited a low detection limit of 0.01 μM, with a linear dynamic range from 0.1 to 10 μM in milk, and a good selectivity toward Neomycin B. Specifically, because of the shorter RNA fragments and the nuclease inhibition ability of the RNA-modified AuNPs, the RNA sequences remained stable during the experiments. This work will serve as an example for the development of novel biosensors based on RNA aptamers.Graphical AbstractAn RNA aptamer-based nanoparticle sensor, developed for the detection of Neomycin B in milk, shows high binding affinity and selectivity. This aptasensor depends on the self-assembly of the aptamer/ligand complex and fluorescence quenching by gold nanoparticles (AuNPs). Because of the shorter RNA fragments and the nuclease inhibition ability of RNA-modified AuNPs, RNA sequences remain stable during the detection.
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DOI：
10.1016/j.bbrc.2012.01.127
发表时间：
2012-03-02
影响因子：
3.1
作者：
Foerster, Charlotte;Zydek, Martin;Fuerste, Jens P.
通讯作者：
Fuerste, Jens P.
DOI：
10.1021/la400617u
发表时间：
2013-05
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Xu Zhang;Biwu Liu;M. Servos;Juewen Liu
通讯作者：
Xu Zhang;Biwu Liu;M. Servos;Juewen Liu
影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
--
作者：
WALLIS, MG;VONAHSEN, U;FAMULOK, M
通讯作者：
FAMULOK, M
影响因子：
12.6
作者：
de-los-Santos-Alvarez, Noemi;Lobo-Castanon, Maria Jesus;Tunon-Blanco, Paulino
通讯作者：
Tunon-Blanco, Paulino