Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells.
Comparative Analysis of Immunohistochemical Staining Intensity Determined by Light Microscopy, ImageJ and QuPath in Placental Hofbauer Cells.
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DOI：
10.1267/ahc.20-00032
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发表时间：
2021-02-25
影响因子：
2.4
通讯作者：
Tauber Z
中科院分区：
文献类型：
--
作者：
Cizkova K;Foltynkova T;Gachechiladze M;Tauber Z
基于软件的免疫组织化学染色分析旨在获得定量、可重复和客观的数据。然而，通常只有特定类型的阳性细胞或结构需要被量化，因此不能执行整个图像分析。Hofbauer胎盘细胞就是这样一个例子，它与滋养层细胞一起显示某些抗原阳性，但只有Hofbauer细胞代表感兴趣的区域（ROI）。两名独立观察员通过ImageJ、QuPath和光学显微镜评价了胎盘样本中Hofbauer细胞的细胞质抗原染色免疫组织化学染色强度。因此，精确的人工测定ROI，即霍夫堡尔细胞，是必要的。我们检测到染色强度的低观察者间变异性。ImageJ和QuPath的观察者之间几乎完全一致，而光学显微镜评价则基本一致。对于ImageJ、QuPath和光学显微镜的比较，所有三种方法的一致性（相同的免疫组织化学强度）达到38.1%的样本。ImageJ和QuPath之间的染色强度几乎完全一致，并且光学显微镜与两种软件的比较具有中等一致性。软件分析要耗时得多，因此，它们的利用至少是值得怀疑的，以评估投资回报率与选择。
Software based analyses of immunohistochemical staining are designed for obtaining quantitative, reproducible, and objective data. However, often times only a certain type of positive cells or structures need to be quantified thus whole image analysis cannot be performed. Such an example is Hofbauer placental cells, which show positivity of some antigens together with trophoblast, but only Hofbauer cells represent the regions of interest (ROIs). Two independent observers evaluated the immunohistochemical staining intensity of Hofbauer cells in placenta samples stained for cytoplasmic antigens by ImageJ, QuPath and light microscopy. Thus, the precise manual determination of ROIs, i.e. Hofbauer cells, was necessary. We detected low inter-observer variability in staining intensity. Almost perfect agreement between observers was reached for ImageJ and QuPath whilst substantial agreement was reached for light microscopy evaluation. As for the comparison of ImageJ, QuPath and light microscopy, the agreement of all three methods (identical immunohistochemical intensity) was achieved for 38.1% samples. The almost perfect agreement of staining intensities was reached between ImageJ and QuPath, and moderate agreement for comparison of the light microscopy to both software. Software analyses are much more time-consuming, thus their utilization is at least questionable to evaluate ROIs with selection.
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影响因子：
4.6
作者：
Bankhead P;Loughrey MB;Fernández JA;Dombrowski Y;McArt DG;Dunne PD;McQuaid S;Gray RT;Murray LJ;Coleman HG;James JA;Salto-Tellez M;Hamilton PW
通讯作者：
Hamilton PW
免疫组织化学自动定量评分中多光谱成像的效用。
影响因子：
3.4
作者：
Fiore C;Bailey D;Conlon N;Wu X;Martin N;Fiorentino M;Finn S;Fall K;Andersson SO;Andren O;Loda M;Flavin R
通讯作者：
Flavin R
影响因子：
2.6
作者：
Fedchenko N;Reifenrath J
通讯作者：
Reifenrath J
影响因子：
7.3
作者：
Bellemare, Judith;Rouleau, Melanie;Guillemette, Chantal
通讯作者：
Guillemette, Chantal
影响因子：
6.6
作者：
O'Hurley G;Sjöstedt E;Rahman A;Li B;Kampf C;Pontén F;Gallagher WM;Lindskog C
通讯作者：
Lindskog C