Molecular Characterization of a Lysozyme Gene and Its Altered Expression Profile in Crowded Beet Webworm (Loxostege sticticalis).
Molecular Characterization of a Lysozyme Gene and Its Altered Expression Profile in Crowded Beet Webworm (Loxostege sticticalis).
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拥挤甜菜网虫 (Loxostege sticticalis) 溶菌酶基因的分子特征及其表达谱的改变
DOI：
10.1371/journal.pone.0161384
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Luo L
中科院分区：
文献类型：
--
作者：
Kong H;Lv M;Mao N;Wang C;Cheng Y;Zhang L;Jiang X;Luo L
越来越多的证据表明，生活在高密度种群中的昆虫表现出免疫功能的增强，以对抗更高的疾病风险。这种现象被称为密度依赖性预防，已经在许多昆虫物种中进行了实验测试。尽管密度依赖性预防在表现出密度依赖性相多型性的昆虫中特别普遍，但其分子机制仍不清楚。我们以前的研究表明，溶菌酶的抗菌活性是重要的甜菜夜蛾在这一过程中。本研究从L. sticticalis的克隆和表征。该基因全长1078 bp，开放阅读框为426 bp，编码142个氨基酸。该蛋白具有典型的c型溶菌酶的结构特征，并与其他鳞翅目昆虫的c型溶菌酶聚簇。发现LsLysozyme在整个发育阶段都有表达，在蛹中表达水平最高。LsLysozyme在中肠和脂肪体中也有高表达。在L. sticticalis幼虫感染球孢白僵菌和幼虫饲养在拥挤的条件下。此外，与以每罐1或30只幼虫的密度饲养的那些相比，以每罐10只幼虫的密度饲养的感染幼虫中的LsLysozyme的表达水平显著更高。这些结果表明，幼虫拥挤影响这种溶菌酶的基因表达谱。这项研究提供了额外的见解表达的免疫相关的溶菌酶基因，并帮助我们更好地了解免疫反应的L。在拥挤的条件下。
There is growing evidence that insects living in high-density populations exhibit an increase in immune function to counter a higher risk of disease. This phenomenon, known as density-dependent prophylaxis, has been experimentally tested in a number of insect species. Although density-dependent prophylaxis is especially prevalent in insects exhibiting density-dependent phase polyphenism, the molecular mechanism remains unclear. Our previous study demonstrated that the antibacterial activity of lysozyme is important for this process in the beet webworm Loxostege sticticalis. In this study, a lysozyme cDNA from L. sticticalis was cloned and characterized. The full-length cDNA is 1078 bp long and contains an open reading frame of 426 bp that encodes 142 amino acids. The deduced protein possesses structural characteristics of a typical c-type lysozyme and clusters with c-type lysozymes from other Lepidoptera. LsLysozyme was found to be expressed throughout all developmental stages, showing the highest level in pupae. LsLysozyme was also highly expressed in the midgut and fat body. Elevated LsLysozyme expression was observed in L. sticticalis larvae infected by Beauveria bassiana and in larvae reared under crowding conditions. In addition, the expression level of LsLysozyme in infected larvae reared at a density of 10 larvae per jar was significantly higher compared to those reared at a density of l or 30 larvae per jar. These results suggest that larval crowding affects the gene expression profile of this lysozyme. This study provides additional insight into the expression of an immune-associated lysozyme gene and helps us to better understand the immune response of L. sticticalis under crowding conditions.
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影响因子：
2.9
作者：
Lapcharoen, Parichat;Komalamisra, Narumon;Rongsriyam, Yupha;Wangsuphachart, Voranuch;Dekumyoy, Paron;Prachumsri, Jetsumon;Kajla, Mayur K.;Paskewitz, Susan M.
通讯作者：
Paskewitz, Susan M.
影响因子：
2.8
作者：
Kwon, Hyojung;Bang, Kyeongrin;Cho, Saeyoull
通讯作者：
Cho, Saeyoull
影响因子：
3.8
作者：
Regel, R;Matioli, SR;Terra, WR
通讯作者：
Terra, WR
影响因子：
5.6
作者：
MAENAKA, K;MATSUSHIMA, M;KUMAGAI, I
通讯作者：
KUMAGAI, I
影响因子：
3.7
作者：
Silva FW;Viol DL;Faria SV;Lima E;Valicente FH;Elliot SL
通讯作者：
Elliot SL