Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli.
Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli.
复制标题
DOI：
10.1016/j.ymben.2012.05.001
复制
发表时间：
2012-09
影响因子：
8.4
通讯作者：
Georgiou, George
中科院分区：
文献类型：
--
作者：
Skretas, Georgios;Makino, Tomohiro;Varadarajan, Navin;Pogson, Mark;Georgiou, George
关键词：
Low yields of recombinant expression represent a major barrier to the physical characterization of membrane proteins. Here, we have identified genes that globally enhance the production of properly folded G protein-coupled receptors (GPCRs) in Escherichia coli. Libraries of bacterial chromosomal fragments were screened using two separate systems that monitor: (i) elevated fluorescence conferred by enhanced expression of GPCR-GFP fusions and (ii) increased binding of fluorescent ligand in cells producing more active receptor. Three multi-copy hits were isolated by both methods: nagD, encoding the ribonucleotide phosphatase NagD; a fragment of nlpD, encoding a truncation of the predicted lipoprotein NlpD, and the three-gene cluster ptsN-yhbJ-npr, encoding three proteins of the nitrogen phosphotransferase system. Expression of these genes resulted in a 3- to 10-fold increase in the yields of different mammalian GPCRs. Our data is consistent with the hypothesis that the expression of these genes may serve to maintain the integrity of the bacterial periplasm and to provide a favorable environment for proper membrane protein folding, possibly by inducing a fine-tuned stress response and/or via modifying the composition of the bacterial cell envelope.
登录
查看更多内容
DOI：
10.1126/science.1191652
发表时间：
2010-10-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ajikumar PK;Xiao WH;Tyo KE;Wang Y;Simeon F;Leonard E;Mucha O;Phon TH;Pfeifer B;Stephanopoulos G
通讯作者：
Stephanopoulos G
影响因子：
8.4
作者：
Makino, Tomohiro;Skretas, Georgios;Kang, Tae-Hyun;Georgiou, George
通讯作者：
Georgiou, George
影响因子：
4.8
作者：
Beha, D;Deitermann, S;Koch, HG
通讯作者：
Koch, HG
影响因子：
3.5
作者：
Cormack, BP;Valdivia, RH;Falkow, S
通讯作者：
Falkow, S
影响因子：
9.9
作者：
通讯作者：
--