An accurate method for antigen β-conglycinin detection in soybean meal
An accurate method for antigen β-conglycinin detection in soybean meal
复制标题
豆粕中β-伴大豆球蛋白抗原的准确检测方法
DOI：
10.1016/j.jfca.2022.104861
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发表时间：
2022-08
影响因子：
4.3
通讯作者：
Yu Deng
中科院分区：
文献类型：
--
作者：
LiZhou Ma;Yafeng Wei;Yin Mao;YingYing Liu;Guohui Li;Yu Deng
β-conglycinin is a major antigenic protein in soybeans that can cause allergy, diarrhea, and loss of appetite in young animals, but there are many drawbacks in the existing detection methods. Herein, we built an accurate and sensitive aptamer sensor to rapidly detect the β-conglycinin relying on the specific binding properties of β-conglycinin β subunit and its aptamer. The sensor has excellent performance in terms of sensitivity and detection efficiency that the detection range of β subunit is 7-58 nM, and the detection limit is 2.58 nM. Finally, the additional β subunit in soy milk was successfully verified using the sensor, demonstrating its plug and play properties for complicated conditions. All these are the evidences that aptamer sensor can be used as a novel detection tool for the quality control of soybean meal products. • An effective and precise sensor for detecting soybean antigen protein was built. • 7-58 nM of β subunit could be visual detected, with low detection limit (2.58 nM). • The AuNPs-aptamer sensor is highly specific to β-conglycinin β subunits. • The AuNPs-aptamer sensor can work in complicated conditions.
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