Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
Downregulation of cellular protective factors of rumen epithelium in goats fed high energy diet.
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山羊瘤胃上皮的细胞保护因子的下调喂养了高能量饮食。
DOI：
10.1371/journal.pone.0081602
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zebeli Q
中科院分区：
文献类型：
--
作者：
Hollmann M;Miller I;Hummel K;Sabitzer S;Metzler-Zebeli BU;Razzazi-Fazeli E;Zebeli Q
高能量日粮可挑战瘤胃上皮细胞的代谢和保护功能，但其潜在因素尚不清楚。本研究旨在评估蛋白质组学的变化，山羊瘤胃上皮细胞在低，中，高能量饮食。蛋白质表达的变化进行了比较，通过二维差异凝胶电泳，然后用基质辅助激光解吸电离串联飞行时间质谱进行蛋白质鉴定。在所有凝胶中通常检测到的约2，000个点中，有64个点受到显著调节，这些点可以追溯到24种独特的蛋白质。有趣的是，表达谱的几个分子伴侣蛋白具有重要的细胞保护功能，如热休克同源71 kDa蛋白，过氧化物酶氧还蛋白-6，丝氨酸蛋白酶抑制剂H1，蛋白质二硫键异构酶，硒结合蛋白集体下调响应高饮食能量供应。类似的调控模式，获得了一些其他蛋白质参与运输或代谢功能。相反，代谢酶如视网膜脱氢酶1和ATP合酶亚基β，线粒体前体在高能量饮食中上调。瘤胃上皮细胞中伴侣蛋白的表达较低，以响应高能量供应可能表明，这些细胞对潜在有害的瘤胃有毒化合物的保护较少，这可能会对瘤胃和全身健康产生影响。我们的研究结果还表明，富含能量的饮食和由此产生的酸损伤可能会使瘤胃上皮细胞更容易受到细胞损伤，削弱他们的细胞防御系统，从而促进瘤胃屏障功能的损害，通常在富含能量的反刍动物饲养。
Energy-rich diets can challenge metabolic and protective functions of the rumen epithelial cells, but the underlying factors are unclear. This study sought to evaluate proteomic changes of the rumen epithelium in goats fed a low, medium, or high energy diet. Expression of protein changes were compared by two-dimensional differential gel electrophoresis followed by protein identification with matrix assisted laser desorption ionisation tandem time-of-flight mass spectrometry. Of about 2,000 spots commonly detected in all gels, 64 spots were significantly regulated, which were traced back to 24 unique proteins. Interestingly, the expression profiles of several chaperone proteins with important cellular protective functions such as heat shock cognate 71 kDa protein, peroxiredoxin-6, serpin H1, protein disulfide-isomerase, and selenium-binding protein were collectively downregulated in response to high dietary energy supply. Similar regulation patterns were obtained for some other proteins involved in transport or metabolic functions. In contrast, metabolic enzymes like retinal dehydrogenase 1 and ATP synthase subunit beta, mitochondrial precursor were upregulated in response to high energy diet. Lower expressions of chaperone proteins in the rumen epithelial cells in response to high energy supply may suggest that these cells were less protected against the potentially harmful rumen toxic compounds, which might have consequences for rumen and systemic health. Our findings also suggest that energy-rich diets and the resulting acidotic insult may render rumen epithelial cells more vulnerable to cellular damage by attenuating their cell defense system, hence facilitating the impairment of rumen barrier function, typically observed in energy-rich fed ruminants.
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DOI：
10.1152/ajpregu.00597.2011
发表时间：
2012-11-01
影响因子：
2.8
作者：
Kuzinski, Judith;Zitnan, Rudolf;Schweigel-Roentgen, Monika
通讯作者：
Schweigel-Roentgen, Monika
影响因子：
3.5
作者：
Bhat, PV
通讯作者：
Bhat, PV
影响因子：
3.3
作者：
Bjarnadottir, S. G.;Hollung, K.;Veiseth-Kent, E.
通讯作者：
Veiseth-Kent, E.
影响因子：
3.5
作者：
Emmanuel, D. G. V.;Madsen, K. L.;Ametaj, B. N.
通讯作者：
Ametaj, B. N.
影响因子：
5.3
作者：
HIRAYOSHI, K;KUDO, H;NAGATA, K
通讯作者：
NAGATA, K