A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum.
A putative high affinity phosphate transporter, CmPT1, enhances tolerance to Pi deficiency of chrysanthemum.
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一种推定的高亲和力磷酸盐转运蛋白 CmPT1 可增强菊花对 Pi 缺乏的耐受性
DOI：
10.1186/1471-2229-14-18
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发表时间：
2014-01-10
影响因子：
5.3
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Liu P;Chen S;Song A;Zhao S;Fang W;Guan Z;Liao Y;Jiang J;Chen F
背景无机磷（Pi）是植物生长所必需的营养元素，缺磷是植物生长发育的主要限制因子。它的获得主要是由Pht1转运蛋白介导的，Pht1转运蛋白是一个位于质膜上的蛋白家族。菊花是一种重要的观赏植物，在缺磷的土壤中生长会影响其生产力，但对菊花中磷转运蛋白的研究还很有限.结果从菊花中分离到一个磷酸盐转运蛋白1（Phosphate Transporter 1，PT1）家族的同源蛋白，其预测产物是一个具有12个跨膜结构域的蛋白，与其它高亲和力植物磷转运蛋白高度同源.实时定量PCR分析表明，该基因的转录强烈的根，弱的茎和低于检测水平的菊花植物生长在足够或缺乏Pi的条件下的叶子。转录本丰度大大提高了Pi饥饿的根。酵母中的互补测定表明，CmPT1部分补偿了酵母菌株MB192中磷酸盐转运蛋白活性的缺失。CmPT 1的估计Km为35.2 μM。结论CmPT1基因编码一种典型的、根表达的、高亲和力的磷酸盐转运蛋白，在菊花磷胁迫中发挥重要作用。
BackgroundInorganic phosphate (Pi) is essential for plant growth, and phosphorus deficiency is a main limiting factor in plant development. Its acquisition is largely mediated by Pht1 transporters, a family of plasma membrane-located proteins. Chrysanthemum is one of the most important ornamental plants, its productivity is usually compromised when grown in phosphate deficient soils, but the study of phosphate transporters in chrysanthemum is limited.ResultsWe described the isolation from chrysanthemum of a homolog of thePhosphate Transporter 1(PT1) family.Its predicted product is a protein with 12 transmembrane domains, highly homologous with other high affinity plant Pi transporters. Real-time quantitative PCR analysis revealed that the gene was transcribed strongly in the root, weakly in the stem and below the level of detection in the leaf of chrysanthemum plants growing in either sufficient or deficient Pi conditions. Transcript abundance was greatly enhanced in Pi-starved roots. A complementation assay in yeast showed that CmPT1 partially compensated for the absence of phosphate transporter activity in yeast strain MB192. The estimatedKmof CmPT1 was 35.2 μM. Under both Pi sufficient and deficient conditions, transgenic plants constitutively expressingCmPT1grew taller than the non-transformed wild type, produced a greater volume of roots, accumulated more biomass and took up more phosphate.ConclusionsCmPT1encodes a typical, root-expressed, high affinity phosphate transporter, plays an important role in coping Pi deficiency of chrysanthemum plants.
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磷酸盐转运蛋白基因 OsPht1;8 参与水稻磷酸盐稳态
影响因子：
7.4
作者：
Jia, Hongfang;Ren, Hongyan;Xu, Guohua
通讯作者：
Xu, Guohua
DOI：
10.1073/pnas.96.10.5868
发表时间：
1999-05-11
影响因子：
11.1
作者：
Muchhal, US;Raghothama, KG
通讯作者：
Raghothama, KG
DOI：
10.1073/pnas.93.19.10519
发表时间：
1996-09-17
影响因子：
11.1
作者：
Muchhal, US;Pardo, JM;Raghothama, KG
通讯作者：
Raghothama, KG
影响因子：
3.9
作者：
Nagy, R;Vasconcelos, MJV;Bucher, M
通讯作者：
Bucher, M
影响因子：
2.1
作者：
JONES, JB
通讯作者：
JONES, JB