Sensitivity of bovine corpora lutea to prostaglandin F2alpha is dependent on progesterone, oxytocin, and prostaglandins.
Sensitivity of bovine corpora lutea to prostaglandin F2alpha is dependent on progesterone, oxytocin, and prostaglandins.
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牛黄体对前列腺素 F2α 的敏感性取决于孕酮、催产素和前列腺素。
作者：
D. Skarżyński;K. Okuda
从子宫释放的前列腺素（PG）F2 α对牛的自发性黄体溶解至关重要。尽管PGF 2 α及其类似物被广泛用于通过诱导黄体溶解来同步动情周期，但动情周期早期的黄体（CL）对PGF 2 α的黄体溶解作用具有抵抗力。我们研究了牛CL对体外PGF 2 α治疗的敏感性，并确定CL对PGF 2 α的反应变化是否依赖于孕酮（P4）、催产素（OT）和局部产生的PG。在用PGF 2 α刺激之前，将早期（发情周期第4-5天）和中期CL（发情周期第8-12天）的牛黄体细胞预先暴露于P4拮抗剂（奥那司酮：OP; 10（-4）M）、OT拮抗剂（阿托西班：AT; 10（-6）M）或吲哚美辛（INDO; 10（-4）M）12 h。虽然OP仅在早期CL中减少P4分泌（p < 0.001），但在所检查的两个阶段的细胞中它减少OT分泌（p < 0.001）。OP还减少了早期CL的PGF 2 α和PGE 2分泌（p < 0.01）。然而，它刺激了周期中期黄体细胞的PGF 2 α分泌（p < 0.001）。AT降低早、中期CL的P4分泌（p < 0.05）。AT还可抑制CL早期PGF 2 α的分泌（p < 0.05）。测量OT分泌和细胞内游离Ca 2+（[Ca 2 +]i）水平作为CL对PGF 2 α敏感性的指标。PGF 2 α对OT分泌无影响，但CL早期[Ca 2 +]i升高（p < 0.05）。然而，与对照组相比，OP、AT和INDO预处理后，细胞中PGF 2 α的作用增强（p < 0.01）。在周期中期黄体细胞中，PGF 2 α诱导OT分泌和[Ca 2 +]i增加2倍。然而，与早期CL的结果相反，这些增加仅通过细胞预暴露于AT而放大（p < 0.05）。这些结果表明，黄体P4，OT，和PG的自分泌/旁分泌正反馈级联在牛早中期周期CL的组件，并可能是负责早期牛CL的外源性PGF 2 α作用的阻力。
Prostaglandin (PG) F2alpha that is released from the uterus is essential for spontaneous luteolysis in cattle. Although PGF2alpha and its analogues are extensively used to synchronize the estrous cycle by inducing luteolysis, corpora lutea (CL) at the early stage of the estrous cycle are resistant to the luteolytic effect of PGF2alpha. We examined the sensitivity of bovine CL to PGF2alpha treatment in vitro and determined whether the changes in the response of CL to PGF2alpha are dependent on progesterone (P4), oxytocin (OT), and PGs produced locally. Bovine luteal cells from early (Days 4-5 of the estrous cycle) and mid-cycle CL (Days 8-12 of the estrous cycle) were preexposed for 12 h to a P4 antagonist (onapristone: OP; 10(-4) M), an OT antagonist (atosiban: AT; 10(-6) M), or indomethacin (INDO; 10(-4) M) before stimulation with PGF2alpha. Although OP reduced P4 secretion (p < 0.001) only in early CL, it reduced OT secretion in the cells of both phases examined (p < 0.001). OP also reduced PGF2alpha and PGE2 secretion (p < 0.01) from early CL. However, it stimulated PGF2alpha secretion in mid-cycle luteal cells (p < 0.001). AT reduced P4 secretion in early and mid-cycle CL (p < 0.05). Moreover, PGF2alpha secretion was inhibited (p < 0.05) by AT in early CL. The OT secretion and the intracellular level of free Ca2+ ([Ca2+]i) were measured as indicators of CL sensitivity to PGF2alpha. PGF2alpha had no influence on OT secretion, although [Ca2+]i increased (p < 0.05) in the early CL. However, the effect of PGF2alpha was augmented (p < 0.01) in cells after pretreatment with OP, AT, and INDO in comparison with the controls. In mid-cycle luteal cells, PGF2alpha induced 2-fold increases in OT secretion and [Ca2+]i. However, in contrast to results in early CL, these increases were magnified only by preexposure of the cells to AT (p < 0.05). These results indicate that luteal P4, OT, and PGs are components of an autocrine/paracrine positive feedback cascade in bovine early to mid-cycle CL and may be responsible for the resistance of the early bovine CL to the exogenous PGF2alpha action.
绵羊黄体组织中编码两种蛋白激酶 C 抑制剂的 mRNA 的稳态浓度。
DOI：
10.1530/jrf.0.1130299
发表时间：
1998
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Juengel,JL;Melner,MH;Clapper,JA;Turzillo,AM;Moss,GE;Nett,TM;Niswender,GD
通讯作者：
Niswender,GD
前列腺素 F2α 调节早期和中期牛黄体的明显生理变化。
影响因子：
3.6
作者：
Tsai,SJ;Wiltbank,MC
通讯作者：
Wiltbank,MC
牛黄体细胞在黄体期的三个阶段分泌黄体酮和前列腺素。
DOI：
10.1677/joe.0.1180121
发表时间：
1988
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Rodgers,RJ;Mitchell,MD;Simpson,ER
通讯作者：
Simpson,ER