Fractional Separation and Estimation of Mark-Houwink-Sakurada Equation Parameters for Gluten
Fractional Separation and Estimation of Mark-Houwink-Sakurada Equation Parameters for Gluten
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面筋 Mark-Houwink-Sakurada 方程参数的分数分离和估计
DOI:
10.1515/1556-3758.2667
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发表时间:
2012-05
影响因子:
1.6
通讯作者:
Li, Bing
中科院分区:
文献类型:
--
作者:
Zhao, Lei;Li, Lin;Liu, Guoqin;Chen, Ling;Li, Xiaoxi;Li, Bing
Fractional separation of gluten using the fast protein liquid chromatography (FPLC) and a numerical method for determination of Mark-Houwink-Sakurada (MHS) equation were studied. With 1% SDS buffer as solvent, the gluten was disassembled into two components: SDS soluble (SDS-S-G) and SDS insoluble gluten proteins (SDS-IS-G). The average molecular weight (Mn, Mw and Mz) and intrinsic viscosity [η] were measured by the multi-angle laser light scattering (MALLS) in conjunction with a size-exclusion chromatography (SEC) and viscosimetry. MHS equations for SDS soluble gluten and SDS insoluble gluten proteins were established as: (SDS-S-G) [η]=1.7459×10-2Mv0.6933=1.7459×10-2qMHSMw0.6933=1.6677×10-2Mw0.6933 (SDS-IS-G) [η]=7.5682×10-3Mv0.7323=7.5682×10-3qMHSMw0.7323=7.2079×10-3Mw0.7323 where qMHS was the polydispersity correction factor.
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