A Comparison of the Production of Ethanol between Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation Using Unpretreated Cassava Pulp and Enzyme Cocktail
A Comparison of the Production of Ethanol between Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation Using Unpretreated Cassava Pulp and Enzyme Cocktail
复制标题
使用未经预处理的木薯浆和酶混合物同时糖化和发酵与单独水解和发酵生产乙醇的比较
DOI：
10.1271/bbb.110750
复制
发表时间：
2012-04
影响因子：
1.6
通讯作者：
Jufang WANG
中科院分区：
文献类型：
--
作者：
朱明军;Ping LI;Xinfang GONG;Jufang WANG
采用分离水解发酵（SHF）和同时糖化发酵（SSF）工艺，对木薯浆进行预处理生产乙醇。淀粉酶、纤维素酶、纤维素酶和葡萄糖淀粉酶的组合在SHF和SSF方法中都产生了最高水平的乙醇产量。温度37℃，pH 5.0，接种量6%为SSF的最佳条件。在纸浆浓度为20%的间歇工艺中，SHF和SSF的乙醇产量最高，分别为23.51和34.67 g L−1，但在进料间歇工艺中，SHF和SSF的乙醇产量分别达到29.39和43.25 g L−1，分别比间歇工艺高25%和24.7%。因此，采用进料批法进行SSF为木薯浆的利用提供了一种更有效的方法。
The processes of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) were employed using Saccharomyces cerevisiae for the production of ethanol from cassava pulp without any pretreatment. A combination of amylase, cellulase, cellobiase, and glucoamylase produced the highest levels of ethanol production in both the SHF and the SSF method. A temperature of 37 °C, a pH of 5.0, and an inoculum size of 6% were the optimum conditions for SSF. For the batch process at a pulp concentration of 20%, ethanol production levels from SHF and SSF were the highest, at 23.51 and 34.67 g L−1 respectively, but in the fed-batch process, the levels of ethanol production from SHF and SSF rose to 29.39 and 43.25 g L−1 respectively, which were 25% and 24.7% higher than those of the batch process. Thus SSF using the fed-batch provided a more efficient method for the utilization of cassava pulp.
影响因子：
3.4
作者：
GHOSH, P;PAMMENT, NB;MARTIN, WRB
通讯作者：
MARTIN, WRB