The presence of clonogenic cells in high-grade malignant lymphoma: a prognostic factor.
The presence of clonogenic cells in high-grade malignant lymphoma: a prognostic factor.
复制标题
高级恶性淋巴瘤中克隆细胞的存在：一个预后因素。
作者：
M. Tweeddale;B. Lim;N. Jamal;James Robinson;John R. Zalcberg;G. Lockwood;Minden;Hans A. Messner
已经开发了一种培养系统，其促进了一些患有中、高度恶性淋巴瘤的患者的克隆性淋巴瘤细胞的生长。人血浆最能支持骨髓、淋巴结和外周血样本中的集落形成。一些患者的集落形成取决于生长因子，在本研究中，生长因子以植物血凝素（PHA）刺激的白细胞（PHA-LCM）条件培养基的形式添加。有些人没有PHA-LCM引起淋巴瘤集落，但与PHA-LCM提高他们的频率，其他人是完全独立的PHA-LCM。将原代培养物中生长的集落常规再克隆并繁殖为具有稳定B细胞表型的EB病毒（EBV）阴性细胞系。细胞系显示出与原发性淋巴瘤样品中观察到的相同的免疫球蛋白重排模式。此外，在培养数据和临床结果之间观察到显著的临床相关性。在其临床过程中的任何时间形成淋巴瘤集落的患者的生存期明显短于未产生集落的患者的生存期（P = 0.0009）。对诊断时研究的患者亚组进行生存评估时，观察结果相同（P = 0.0014）。
A culture system has been developed that promotes growth of clonogenic lymphoma cells of some patients with intermediate and high-grade malignant lymphoma. The formation of colonies in bone marrow, lymph nodes, and peripheral blood samples is best supported by human plasma. Colony formation of some patients was dependent upon growth factors, which in this study were added in the form of medium conditioned by phytohemagglutinin (PHA)-stimulated leukocytes (PHA-LCM). Some gave rise to lymphoma colonies without PHA-LCM but improved their frequency with PHA-LCM; others were completely independent of PHA-LCM. Colonies grown in primary cultures were routinely recloned and propagated as Epstein-Barr virus (EBV)-negative cell lines with stable B cell phenotype. The cell lines showed the same immunoglobulin rearrangement pattern as that observed in the primary lymphoma sample. In addition, a significant clinical correlation was observed between culture data and clinical outcome. Survival of patients who formed lymphoma colonies at any time during their clinical course was significantly shorter than survival of patients who did not give rise to colonies (P = 0.0009). The same observation was made when the survival assessment was performed for the subset of patients studied at diagnosis (P = 0.0014).
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急性淋巴细胞白血病同种异体骨髓移植后，携带 Epstein-Barr 病毒基因组的供体细胞中出现单克隆免疫母细胞肉瘤。
影响因子：
20.3
作者：
Schubach,WH;Hackman,R;Neiman,PE;Miller,G;Thomas,ED
通讯作者：
Thomas,ED
骨髓移植治疗非霍奇金淋巴瘤的回顾。
DOI：
10.1200/jco.1983.1.7.440
发表时间：
1983
期刊：
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子：
--
作者：
Appelbaum,FR;Thomas,ED
通讯作者：
Thomas,ED
弥漫性大细胞淋巴瘤的免疫异质性。
影响因子：
20.3
作者：
Freedman,AS;Boyd,AW;Anderson,KC;Fisher,DC;Pinkus,GS;Schlossman,SF;Nadler,LM
通讯作者：
Nadler,LM
影响因子：
20.3
作者：
ANDERSON, KC;BATES, MP;NADLER, LM
通讯作者：
NADLER, LM
B 细胞生长和分化因子。
影响因子：
--
作者：
Brooks,K;Fernandez,R;Vitetta,ES
通讯作者：
Vitetta,ES