Visceral Adipose Tissue of Prediabetic and Diabetic Females Shares a Set of Similarly Upregulated microRNAs Functionally Annotated to Inflammation, Oxidative Stress and Insulin Signaling.
Visceral Adipose Tissue of Prediabetic and Diabetic Females Shares a Set of Similarly Upregulated microRNAs Functionally Annotated to Inflammation, Oxidative Stress and Insulin Signaling.
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DOI：
10.3390/antiox10010101
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发表时间：
2021-01-12
期刊：
影响因子：
--
通讯作者：
Śliwińska A
中科院分区：
文献类型：
--
作者：
Strycharz J;Wróblewski A;Zieleniak A;Świderska E;Matyjas T;Rucińska M;Pomorski L;Czarny P;Szemraj J;Drzewoski J;Śliwińska A
肥大和缺氧的内脏脂肪组织（VAT）分泌促炎细胞因子，促进胰岛素抵抗（IR）、前驱糖尿病和2型糖尿病（T2 DM）微RNA（miRNA）是代谢紊乱的标志物，其调节对例如，炎症、葡萄糖代谢和抗氧化防御，提高诊断价值。本研究的目的是使用qPCR评估高血糖症是否能够影响糖尿病前期（IFG）和糖尿病（T2 DM）患者与血糖正常（NG）受试者的VAT中所选miRNA的表达。统计学分析表明，miRNAs的表达可能是性别依赖性的。因此，我们确定了NG、T2 DM、IFG女性中差异表达的15种miRNAs（miR-10a-5p、let-7d-5p、miR-532-5p、miR-127-3p、miR-125b-5p、let-7a-5p、let-7e-5p、miR-199a-3p、miR-365a-3p、miR-99a-5p、miR-100-5p、miR-342-3p、miR-146b-5p、miR-127 -3p、miR-125b-5p、miR-125a-5p、miR-125a-5p、miR-125a-5p、miR-125a-5p、miR-125b-5p、miR-125a-5p、miR-125b-5p、miR-125a-5p、miR-125b-5p、miR-125b-5p、miR-125b-5p）miR-204-5p、miR-409-3p）。与NG受试者相比，女性T2 DM和IFG患者的增值税中大多数显着变化的miRNA也同样上调，与FPG和HbA 1c正相关，但与WHR/BMI不相关。富集分析表明，11个顶级DE miRNA参与氧化应激，炎症和胰岛素信号传导。这些miRNAs的表达变化可能与高血糖患者VAT中的轻度慢性炎症和氧化应激有关。
Hypertrophic and hypoxic visceral adipose tissue (VAT) secretes proinflammatory cytokines promoting insulin resistance (IR), prediabetes and type 2 diabetes (T2DM) microRNAs (miRNAs) are markers of metabolic disorders regulating genes critical for e.g., inflammation, glucose metabolism, and antioxidant defense, with raising diagnostic value. The aim of the current study was to evaluate whether hyperglycemia is able to affect the expression of selected miRNAs in VAT of prediabetic (IFG) and diabetic (T2DM) patients vs. normoglycemic (NG) subjects using qPCR. Statistical analyses suggested that miRNAs expression could be sex-dependent. Thus, we determined 15 miRNAs as differentially expressed (DE) among NG, T2DM, IFG females (miR-10a-5p, let-7d-5p, miR-532-5p, miR-127-3p, miR-125b-5p, let-7a-5p, let-7e-5p, miR-199a-3p, miR-365a-3p, miR-99a-5p, miR-100-5p, miR-342-3p, miR-146b-5p, miR-204-5p, miR-409-3p). Majority of significantly changed miRNAs was similarly upregulated in VAT of female T2DM and IFG patients in comparison to NG subjects, positively correlated with FPG and HbA1c, yet, uncorrelated with WHR/BMI. Enrichment analyses indicated involvement of 11 top DE miRNAs in oxidative stress, inflammation and insulin signaling. Those miRNAs expression changes could be possibly associated with low-grade chronic inflammation and oxidative stress in VAT of hyperglycemic subjects.
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DOI：
10.1002/oby.21950
发表时间：
2017-10
期刊：
Obesity (Silver Spring, Md.)
影响因子：
--
作者：
Jones A;Danielson KM;Benton MC;Ziegler O;Shah R;Stubbs RS;Das S;Macartney-Coxson D
通讯作者：
Macartney-Coxson D
影响因子：
3.3
作者：
Li, Ping;Fan, Cunxia;Guan, Meiping
通讯作者：
Guan, Meiping
影响因子：
4.4
作者：
Link JC;Hasin-Brumshtein Y;Cantor RM;Chen X;Arnold AP;Lusis AJ;Reue K
通讯作者：
Reue K
影响因子：
3.4
作者：
Li YB;Wu Q;Liu J;Fan YZ;Yu KF;Cai Y
通讯作者：
Cai Y
影响因子：
3.7
作者：
Chartoumpekis DV;Zaravinos A;Ziros PG;Iskrenova RP;Psyrogiannis AI;Kyriazopoulou VE;Habeos IG
通讯作者：
Habeos IG