Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
Differential expression of microRNAs in adipose tissue after long-term high-fat diet-induced obesity in mice.
复制标题
DOI：
10.1371/journal.pone.0034872
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Habeos IG
中科院分区：
文献类型：
--
作者：
Chartoumpekis DV;Zaravinos A;Ziros PG;Iskrenova RP;Psyrogiannis AI;Kyriazopoulou VE;Habeos IG
肥胖是全球主要的健康问题，它与代谢综合征、心血管疾病和癌症等慢性疾病的风险增加有关。阐明参与脂肪形成和肥胖形成的分子机制是至关重要的，因为它可以导致识别新的生物标志物和治疗靶点，用于开发抗肥胖药物。microRNA（miRNAs）在多种生物学过程中发挥着重要的调控作用。它们已成为一个不断增长的研究领域，包括有前途的药物靶点在各个领域，如癌症，代谢等。本研究调查了可能的含义，脂肪组织中的miRNAs在肥胖症的发展过程中使用作为模型的C57 BLJ 6小鼠喂食高脂肪饮食。将C57 BLJ 6野生型雄性小鼠饲喂标准（SD）或高脂肪饮食（HFD）5个月。从白色脂肪组织制备总RNA，并用于微RNA谱分析和qPCR。使用qPCR验证了通过microRNA谱鉴定的22种最差异表达的miRNA。本研究的结果证实了以前的结果。先前也报道了HFD喂养后mmu-miR-222的上调和mmu-miR-200 b、mmu-miR-200 c、mmu-miR-204、mmu-miR-30 a *、mmu-miR-193、mmu-miR-378和mmu-miR-30 e * 的下调。另一方面，我们首次显示了mmu-miR-342- 3 p、mmu-miR-142 - 3 p、mmu-miR-142- 5 p、mmu-miR-21、mmu-miR-146 a、mmu-miR-146 b、mmu-miR-379的上调和mmu-miR-122、mmu-miR-133 b、mmu-miR-1、mmu-miR-30 a *、mmu-miR-192和mmu-miR-203在肥胖发展过程中的作用。然而，为了了解miRNAs在脂肪形成和肥胖中的确切作用，未来的研究是必要的。
Obesity is a major health concern worldwide which is associated with increased risk of chronic diseases such as metabolic syndrome, cardiovascular disease and cancer. The elucidation of the molecular mechanisms involved in adipogenesis and obesogenesis is of essential importance as it could lead to the identification of novel biomarkers and therapeutic targets for the development of anti-obesity drugs. MicroRNAs (miRNAs) have been shown to play regulatory roles in several biological processes. They have become a growing research field and consist of promising pharmaceutical targets in various fields such as cancer, metabolism, etc. The present study investigated the possible implication of miRNAs in adipose tissue during the development of obesity using as a model the C57BLJ6 mice fed a high-fat diet. C57BLJ6 wild type male mice were fed either a standard (SD) or a high-fat diet (HFD) for 5 months. Total RNA was prepared from white adipose tissue and was used for microRNA profiling and qPCR. Twenty-two of the most differentially expressed miRNAs, as identified by the microRNA profiling were validated using qPCR. The results of the present study confirmed previous results. The up-regulation of mmu-miR-222 and the down-regulation of mmu-miR-200b, mmu-miR-200c, mmu-miR-204, mmu-miR-30a*, mmu-miR-193, mmu-miR-378 and mmu-miR-30e* after HFD feeding has also been previously reported. On the other hand, we show for the first time the up-regulation of mmu-miR-342-3p, mmu-miR-142-3p, mmu-miR-142-5p, mmu-miR-21, mmu-miR-146a, mmu-miR-146b, mmu-miR-379 and the down-regulation of mmu-miR-122, mmu-miR-133b, mmu-miR-1, mmu-miR-30a*, mmu-miR-192 and mmu-miR-203 during the development of obesity. However, future studies are warranted in order to understand the exact role that miRNAs play in adipogenesis and obesity.
登录
查看更多内容
影响因子：
5.8
作者：
Alexander R;Lodish H;Sun L
通讯作者：
Sun L
DOI：
10.1152/ajpendo.90964.2008
发表时间：
2009-05-01
影响因子：
5.1
作者：
Huang, Zhi Hua;Gu, DeSheng;Mazzone, Theodore
通讯作者：
Mazzone, Theodore
脂肪间充质干细胞分化和肥胖小鼠模型中microRNA的全基因组分析。
影响因子：
3.7
作者：
Bengestrate L;Virtue S;Campbell M;Vidal-Puig A;Hadaschik D;Hahn P;Bielke W
通讯作者：
Bielke W
影响因子：
20.3
作者：
Chen, Yun;Gorski, David H.
通讯作者：
Gorski, David H.
影响因子：
4.1
作者：
Chang, Jinhong;Nicolas, Emmanuelle;Taylor, John M
通讯作者：
Taylor, John M