Active microrheology of protein condensates using colloidal probe-AFM.
Active microrheology of protein condensates using colloidal probe-AFM.
复制标题
使用胶体探针 AFM 进行蛋白质凝聚物的主动微流变学。
DOI：
10.1016/j.jcis.2022.11.071
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发表时间：
2022
影响因子：
9.9
通讯作者：
R. de Vries
中科院分区：
文献类型：
--
作者：
Xiufeng Li;J. van der Gucht;P. Erni;R. de Vries
长期以来，人们一直在研究液液相分离产生的蛋白质凝聚物作为生物粘合剂和涂层材料的各种应用。最近，它们也被仔细研究作为细胞中无膜细胞器的模型。在微米尺度上量化它们的界面力学和流变学对于更好地理解细胞中无膜细胞器的物理特性以及开发和改进蛋白质凝聚体的技术应用至关重要。这项研究展示了如何使用带有振荡尖端的胶体探针原子力显微镜同时研究微观尺度蛋白质凝聚物的界面力学和动态流变特性，这些凝聚物是通过精心控制的毛细管凝聚形成的。这种新方法可以访问1到104rad/s的振荡频率。通过等效力学模型分析数据，发现微观尺度蛋白质凝析物力学的三个特征频域：低频时界面张力主导域，中频时过渡域（粘弹性交叉），高频时弹性主导域，涵盖了蛋白质凝析物生物学和技术应用相关的广泛时间尺度。
Protein condensates resulting from liquid–liquid phase separation have long been studied as bio-adhesives and coating materials for various applications. More recently, they are also being scrutinized as models for membraneless organelles in cells. Quantifying their interfacial mechanics and rheology at micrometer scales is vital for better understanding the physics underlying membraneless organelles in cells and for developing and improving technological applications of protein condensates. This study demonstrates how colloidal probe atomic force microscopy with an oscillating tip can be used to simultaneously investigate the interfacial mechanics and dynamic rheological properties of micro-scale protein condensates, formed via carefully controlled capillary condensation. This new approach can access oscillation frequencies ranging from 1 to 104rad/s. By analyzing the data using an equivalent mechanical model, three characteristic frequency domains for the mechanics of micro-scale protein condensates are found: an interfacial tension-dominated domain at low frequencies, a transition domain (viscous-to-elastic crossover) at intermediate frequencies, and an elasticity-dominated domain at high frequencies, covering a broad range of time scales relevant in biology and technological applications of protein condensates.
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影响因子：
21.8
作者：
Wei MT;Elbaum-Garfinkle S;Holehouse AS;Chen CC;Feric M;Arnold CB;Priestley RD;Pappu RV;Brangwynne CP
通讯作者：
Brangwynne CP
影响因子：
5.6
作者：
Seydoux G
通讯作者：
Seydoux G
影响因子：
7.015
作者：
Robertson-Anderson, Rae M.
通讯作者：
Robertson-Anderson, Rae M.
影响因子：
15.6
作者：
Stewart RJ;Wang CS;Shao H
通讯作者：
Shao H