Effects of cage and floor rearing system on the factors of antioxidant defense and inflammatory injury in laying ducks
Effects of cage and floor rearing system on the factors of antioxidant defense and inflammatory injury in laying ducks
复制标题
笼养和地板饲养系统对蛋鸭抗氧化防御和炎症损伤因素的影响
DOI：
10.1186/s12863-019-0806-0
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发表时间：
2019-12
期刊：
影响因子：
2.9
通讯作者：
卢立志
中科院分区：
文献类型：
--
作者：
张扬;顾天天;田勇;陈黎;李国勤;周玮;刘国发;吴信生;曾涛;徐琪;陈国宏;卢立志
笼养蛋鸭作为一种新型的养殖方式，不仅从根本上解决了养鸭业的污染问题，提高了产品的生物安全性和质量，而且与地面饲养相比，实行标准化生产，具有更高的效益。当然，这种制度也带来了一些福利问题和压力伤害的蛋鸡由于缺乏水环境和有限的活动在笼子里。然而，在早期笼stage.ResultsIn这项研究中，80绍兴蛋鸡饲养在地板上，从12周龄的笼子里的抗氧化防御和炎症损伤的因素的影响还没有很好的理解。分别在饲养1、2、4、7、10 d后，对鸭的抗氧化防御和炎症损伤因素进行了研究。结果表明，笼养鸭在刚入笼时就出现了一定程度的肝损伤。抗氧化酶活性分析表明，不同饲养制度对鸭抗氧化能力的变化没有影响，但在日龄变化过程中，2、7、10日龄鸭肝脏丙二醛（MDA）含量显著高于1日龄鸭，而过氧化氢酶（CAT）活性则相反。实时荧光定量PCR（qRT-RCR）结果显示，笼养鸭内质网应激相关基因（CHOP和GRP 78）的相对mRNA水平显著高于地面饲养鸭。此外，环氧化酶2（考克斯2）、一氧化氮合酶（iNOS）、白细胞介素1 β（IL-1β）、白细胞介素2（IL-2）和白细胞介素6（IL-6）等炎性细胞因子的mRNA水平在笼养蛋鸡中也显著升高。这不仅造成了一定程度的肝组织损伤，而且导致了炎症损伤因子的表达增加。因此，我们建议在饲料中添加抗应激剂，以缓解早期笼养阶段的应激。
BackgroundCage-rearing in laying ducks, as a novel rearing system, not only fundamentally solves the pollution problem of the duck industry and improve bio-safety and product quality but also exhibits more benefits by implementing standardized production compared with the floor-rearing. Of course, this system also brings some welfare problems and stress injuries to layers due to lack of water environment and limited activities in the cages. However, the effects on the factors of antioxidant defense and inflammatory injury in the early cage stage are not well-understood.ResultsIn this study, eighty Shaoxing layers were reared on floor and in cages from 12 weeks of age. The ducks were caged 1, 2, 4, 7, and 10 days, the factors of antioxidant defense and inflammatory injury were investigated. The results showed that the caged ducks suffered liver injury to a certain extent when the ducks were just put into the cages. Analysis of antioxidant enzyme activities indicated that the different rearing system could not affect the change of antioxidant capacities, while the liver malondialdehyde (MDA) level was significant higher in the 2-d, 7-d, and 10-d ducks compared with the 1-d ducks during the change of days, while catalase (CAT) activity showed the opposite results. Additionally, quantitative real-time PCR (qRT-RCR) revealed that the relative mRNA levels of endoplasmic reticulum (ER) stress-related gene (CHOP and GRP78) were significantly upregulated in cage rearing ducks compared to that of the floor rearing ducks. Moreover, the mRNA levels of inflammatory cytokines including cycloxygenase-2 (COX-2), nitric oxide synthase (iNOS), Interleukin 1 beta (IL-1β), Interleukin 2 (IL-2) and Interleukin 6 (IL-6), were also increased significantly in caged layers.ConclusionsTaken together, although antioxidant defense has no obvious effect on cage stress, the stress levels of laying ducks vary greatly in the early cage stage, which not only caused liver tissue damage to some extent, but also resulted in increases in the expression of the factors of inflammatory injury. Therefore, we recommend that anti-stress agents should be added in the feed to alleviate the stress in the early cage stage.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
10.1039/c6ra27730f
发表时间：
2017-01
期刊：
RSC
影响因子：
--
作者：
Danning Xu;Wanyan Li;Bingxin Li;Yunbo Tian
通讯作者：
Yunbo Tian
影响因子：
4.4
作者：
G-Y Tan;L. Yang;Y. Fu;J.H. Feng;M.H. Zhang
通讯作者：
G-Y Tan;L. Yang;Y. Fu;J.H. Feng;M.H. Zhang
影响因子：
3.3
作者：
Qu, H.;Yan, H.;Ajuwon, K. M.
通讯作者：
Ajuwon, K. M.
影响因子：
3.5
作者：
Kitamura, Masanori;Hiramatsu, Nobuhiko
通讯作者：
Hiramatsu, Nobuhiko