Comparative physiological and metabolomics analysis reveals that single-walled carbon nanohorns and ZnO nanoparticles affect salt tolerance in Sophora alopecuroides
Comparative physiological and metabolomics analysis reveals that single-walled carbon nanohorns and ZnO nanoparticles affect salt tolerance in Sophora alopecuroides
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比较生理学和代谢组学分析表明单壁碳纳米角和ZnO纳米粒子影响苦豆子的耐盐性
DOI：
10.1039/d0en00582g
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发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Xu Jin
中科院分区：
文献类型：
--
作者：
Wan Jinpeng;Wang Ruling;Bai Hanrui;Wang Yibo;Xu Jin
盐胁迫是制约作物产量和品质的因素之一，随着盐渍化程度的提高，盐胁迫在世界范围内受到越来越多的关注。在这里，我们发现单壁碳纳米角（SWCNHs）和ZnO纳米颗粒（ZnO NPs）代谢组学谱的变化有助于苦豆子幼苗的耐盐性。与未处理对照植株相比，叶面喷施SWCNHs增加了根长、根鲜重和叶片可溶性糖含量，而ZnO NPs增加了根鲜重、叶片干重和可溶性糖含量，说明这两种工程纳米材料（ENMs）促进了苦豆子幼苗的生长。盐胁迫下，叶面喷施SWCNHs提高了苦豆子PSII活性、叶片和根系总蛋白含量、叶片可溶性糖含量和叶片铜含量，而ZnO NPs提高了苦豆子苗高、根鲜重、叶片总蛋白含量、叶片和根系可溶性糖含量、叶片锌含量和根系铜含量，从而提高了苦豆子幼苗的耐盐性。代谢组学分析进一步支持了这一观点，即两种enm通过重编程碳/氮代谢，促进糖酵解和TCA循环产生能量，增加不饱和脂肪酸水平以维持盐胁迫下膜的完整性，从而促进盐耐受性。此外，我们发现，虽然SWCNHs和ZnO NPs都能提高alopecuroides幼苗的耐盐性，但这两种ENMs在调节植物生长和胁迫响应方面有着相似而不同的机制。盐胁迫下，氧化锌NPs处理植株叶片和根系代谢物积累量分别是swcnh处理植株的4.83倍和3.7倍，表明氧化锌NPs对苦豆子幼苗耐盐性的调节效果优于swcnh。综上所述，这两种enm的应用为提高植物耐盐性提供了一条新的途径。
Salt stress, which is one of the limiting factors for crop yield and quality, has received more attention with increasing salinization worldwide. Here, we showed that changes in metabolomic profiling by single-walled carbon nanohorns (SWCNHs) and ZnO nanoparticles (ZnO NPs) contributed to salt tolerance in Sophora alopecuroides seedlings. Compared with untreated control plants, foliar spraying with SWCNHs increased the root length, root fresh weight and leaf soluble sugar content, while ZnO NPs increased the root fresh weight, leaf dry weight and soluble sugar content, indicating that the two engineered nanomaterials (ENMs) improved the growth of S. alopecuroides seedlings. Under salt stress, foliar spraying with SWCNHs increased the PSII activity, total protein contents of leaves and roots, leaf soluble sugar content and copper (Cu) content in the leaves, while ZnO NPs increased the plantlet height, root fresh weight, leaf total protein content, soluble sugar contents of leaves and roots, leaf zinc (Zn) content and root Cu content, thereby improving the salt tolerance of S. alopecuroides seedlings. Metabolome analysis further supported the notion that the two ENMs contributed to salt tolerance by reprogramming the carbon/nitrogen metabolism and promoting glycolysis and the TCA cycle to generate energy and increasing the levels of unsaturated fatty acids to maintain membrane integrity under salt stress. Furthermore, we revealed that, although both SWCNHs and ZnO NPs improved salt tolerance in S. alopecuroides seedlings, there were both similar and distinct mechanisms for the two ENMs in modulating plant growth and stress response. Under salt stress, the accumulation of metabolites in the leaves and roots of ZnO NP-treated plants was 4.83 fold and 3.7 fold higher than those in SWCNH-treated plants, respectively, suggesting a better effect of ZnO NPs in modulating the salt tolerance in S. alopecuroides seedlings than SWCNHs. Taken together, these results suggested that the application of the two ENMs provided a novel way to improve salt tolerance in plants.
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影响因子：
2.5
作者：
Dimkpa, Christian O.;McLean, Joan E.;Anderson, Anne J.
通讯作者：
Anderson, Anne J.
DOI：
10.1515/znc-2002-3-417
发表时间：
2002-04
期刊：
Zeitschrift für Naturforschung C
影响因子：
--
作者：
M. Mandre;H. Tullus;J. Klõšeiko
通讯作者：
M. Mandre;H. Tullus;J. Klõšeiko
影响因子：
8.4
作者：
R. Stefan;R. M. Nejem
通讯作者：
R. Stefan;R. M. Nejem
DOI：
10.1186/s40538-019-0169-9
发表时间：
2019-11
影响因子：
6.6
作者：
M. Souri;G. Tohidloo
通讯作者：
M. Souri;G. Tohidloo
影响因子：
7.4
作者：
Allakhverdiev, SI;Sakamoto, A;Murata, N
通讯作者：
Murata, N