Site-specific proteolysis of mini-F plasmid replication protein RepE destroys initiator function and generates an incompatibility substance
Site-specific proteolysis of mini-F plasmid replication protein RepE destroys initiator function and generates an incompatibility substance
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Mini-F质粒复制蛋白RepE的位点特异性蛋白水解破坏起始子功能并产生不相容物质
DOI：
10.1128/jb.174.9.3004-3010.1992
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发表时间：
1992
影响因子：
3.2
通讯作者：
R. Aleff
中科院分区：
文献类型：
--
作者：
B. Kline;G. S. Sandhu;B. Eckloff;R. Aleff
质粒F的复制是由一个具有自身阻遏和启动子功能的质粒特异性Rep蛋白控制的。Rep蛋白的这两个功能被平衡以实现稳定复制的机制尚不清楚，然而，我们在先前的工作中推测可能涉及Rep蛋白的修饰。我们在这里报告了自然蛋白分解的F-RepE蛋白的检测和鉴定。在大肠杆菌染色体DNA存在的情况下，处理后的分子失去了前17个氨基酰基残基和启动子功能，但获得了更高的DNA结合亲和力。当以反式形式供应时，改变的蛋白质充当不亲和性物质，消除了F‘lac的维持。这些发现表明，蛋白质加工有可能对DNA复制的总体控制做出贡献。
Plasmid F replication is controlled by a plasmid-specified Rep protein with both autorepressor and initiator functions. The mechanism by which these two functions of a Rep protein are balanced to achieve stable replication is unknown; however, we speculated in prior work that Rep protein modification could be involved. We report here that naturally proteolyzed F RepE protein has been detected and characterized. The processed molecule lost the first 17 N-terminal aminoacyl residues and initiator function but acquired increased specific DNA-binding affinity in the presence of Escherichia coli chromosomal DNA. When supplied in trans, the altered protein acts as an incompatibility substance and eliminates maintenance of F'lac. These findings indicate that protein processing has the potential to contribute to the overall control of DNA replication.
大肠杆菌 F 质粒的自主控制机制：repE 基因产物的纯化和表征。
影响因子：
14.9
作者：
Masson,L;Ray,DS
通讯作者：
Ray,DS
大肠杆菌 F 质粒的自主控制机制：启动子/阻遏蛋白的不同复合物与其操纵子和 F 质粒复制起点结合。
影响因子：
14.9
作者：
Masson,L;Ray,DS
通讯作者：
Ray,DS