MicroRNA and Putative Target Discoveries in Chrysanthemum Polyploidy Breeding.
MicroRNA and Putative Target Discoveries in Chrysanthemum Polyploidy Breeding.
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菊花多倍体育种中的 MicroRNA 和假定靶点发现
DOI：
10.1155/2017/6790478
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发表时间：
2017
影响因子：
2.9
通讯作者：
Teng N
中科院分区：
文献类型：
--
作者：
Zhang F;Zhao J;Xu S;Fang W;Chen F;Teng N
MicroRNA（miRNAs）是一类长度约为22个核苷酸的内源性非编码RNA分子，通过在转录水平抑制靶基因的转录或在转录后水平抑制翻译，在植物发育中发挥重要作用。为了解miRNAs及其靶基因在菊花多倍体育种中的作用，利用RNA-Seq技术构建了菊花正常胚和异常胚杂交后的3个sRNA文库。结果共鉴定出170个miRNAs，其中有41个是父本染色体加倍杂交中的特异性miRNAs，如miR 169 b、miR 440和miR 528 - 5 p。miR 164 c和miR 159 a在授粉后18 d的正常胚中高表达，提示miR 164 c和miR 159 a在胚胎发育后期起调控作用。miR 172 c仅在授粉后18 d的正常胚中检测到，说明miR 172 c主要介导胚后发育过程中的基因表达，这些基因可能促进胚的成熟。其他miRNA，包括miR 414、miR 2661和miR 5021，可能调节参与生长素反应和能量代谢途径的基因，然后它们共同调节复杂的胚胎发育。
MicroRNAs (miRNAs), around 22 nucleotides (nt) in length, are a class of endogenous and noncoding RNA molecule that play an essential role in plant development, either by suppressing the transcription of target genes at a transcriptional level or inhibiting translation at a posttranscriptional level. To understand the roles of miRNAs and their target genes in chrysanthemum polyploidy breeding, three sRNA libraries of normal and abnormal embryos after hybridization were performed by RNA-Seq. As a result, a total of 170 miRNAs were identified and there are 41 special miRNAs in cross of paternal chromosome doubling, such as miR169b, miR440, and miR528-5p. miR164c and miR159a were highly expressed in a normal embryo at 18 days after pollination, suggesting the regulatory role at the late stage of embryonic development. miR172c was only detected in the normal embryo at 18 days after pollination, which means that miR172c mainly mediates gene expression in postembryonic development and these genes may promote embryo maturation. Other miRNAs, including miR414, miR2661, and miR5021, may regulate the genes participated in pathways of auxin response and energy metabolism; then they regulate the complex embryonic development together.
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影响因子：
1.9
作者：
Deng, Yanming;Teng, Nianjun;Chang, Qingshan
通讯作者：
Chang, Qingshan
影响因子：
20.5
作者：
Chen, Z. Jeffrey
通讯作者：
Chen, Z. Jeffrey
影响因子：
4.6
作者：
Turchi, Luana;Carabelli, Monica;Ruberti, Ida
通讯作者：
Ruberti, Ida
影响因子：
10.5
作者：
Nodine, Michael D.;Bartel, David P.
通讯作者：
Bartel, David P.
DOI：
10.1073/pnas.0403115101
发表时间：
2004-08-24
影响因子：
11.1
作者：
Kurihara, Y;Watanabe, Y
通讯作者：
Watanabe, Y