Chloroplast Genetic Engineering of a Unicellular Green Alga Haematococcus pluvialis with Expression of an Antimicrobial Peptide
Chloroplast Genetic Engineering of a Unicellular Green Alga Haematococcus pluvialis with Expression of an Antimicrobial Peptide
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表达抗菌肽的单细胞绿藻雨生红球藻叶绿体基因工程
DOI：
10.1007/s10126-020-09978-z
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发表时间：
2020-06
影响因子：
3
通讯作者：
Song Qin
中科院分区：
文献类型：
--
作者：
Kang Wang;Yulin Cui;Yinchu Wang;Zhengquan Gao;Tianzhong Liu;Chunxiao Meng;Song Qin
The purpose of this study was to express an antimicrobial peptide in the chloroplast to further develop the plastid engineering of H. pluvialis. Homologous targeting of the 16S-trnI/trnA-23S region and four endogenous regulatory elements, including the psbA promoter, rbcL promoter, rbcL terminator, and psbA terminator in H. pluvialis, were performed to construct a chloroplast transformation vector for H. pluvialis. The expression of codon-optimized antimicrobial peptide piscidin-4 gene (ant1) and selection marker gene (bar, biolaphos resistance gene) in the chloroplast of H. pluvialis was controlled by the rbcL promoter and psbA promoter, respectively. Upon biolistic transformation and selection with phosphinothricin, integration and expression of ant1 in the chloroplast genome were detected using polymerase chain reaction (PCR), southern blotting, and western blotting. Using this method, we successfully expressed antimicrobial peptide piscidin-4 in H. pluvialis. Hence, our results showed H. pluvialis promises as a platform for expressing recombinant proteins for biotechnological applications, which will further contribute to promoting genetic engineering improvement of this strain.
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影响因子：
2.9
作者：
C. L. Gutiérrez;J. Gimpel;C. Escobar;S. Marshall;V. Henríquez
通讯作者：
C. L. Gutiérrez;J. Gimpel;C. Escobar;S. Marshall;V. Henríquez
DOI：
10.1128/genomea.00181-18
发表时间：
2018-03-22
期刊：
Genome announcements
影响因子：
--
作者：
Bauman N;Akella S;Hann E;Morey R;Schwartz AS;Brown R;Richardson TH
通讯作者：
Richardson TH
影响因子：
2.9
作者：
FAN, L;VONSHAK, A;BOUSSIBA, S
通讯作者：
BOUSSIBA, S
影响因子：
4.6
作者：
Ota S;Morita A;Ohnuki S;Hirata A;Sekida S;Okuda K;Ohya Y;Kawano S
通讯作者：
Kawano S
影响因子：
4.6
作者：
Azevedo RSS;de Sousa JR;Araujo MTF;Martins Filho AJ;de Alcantara BN;Araujo FMC;Queiroz MGL;Cruz ACR;Vasconcelos BHB;Chiang JO;Martins LC;Casseb LMN;da Silva EV;Carvalho VL;Vasconcelos BCB;Rodrigues SG;Oliveira CS;Quaresma JAS;Vasconcelos PFC
通讯作者：
Vasconcelos PFC