Variation in sister chromatid exchange frequencies between human and pig whole blood, plasma leukocyte, and mononuclear leukocyte cultures.
Variation in sister chromatid exchange frequencies between human and pig whole blood, plasma leukocyte, and mononuclear leukocyte cultures.
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人和猪全血、血浆白细胞和单核白细胞培养物之间姐妹染色单体交换频率的变化。
DOI：
10.1002/em.2860080406
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
M. Reigosa
中科院分区：
文献类型：
--
作者：
M. Larramendy;M. Reigosa
本文研究了紫外线(UV)对人和猪全血培养(WBC)和血浆白细胞培养(PLC)的姐妹染色单体交换(SCE)的诱导作用。对照组和处理组猪WBC与PLC之间的姐妹染色单体交换频率无明显差异。相反，在对照组和紫外线照射的细胞中，人PLC的姐妹染色单体交换频率始终高于WBC。因此，红细胞并不影响淋巴细胞对紫外光照射的敏感性，人白细胞和PLC诱导SCES的培养条件一定不同(S)，而猪淋巴细胞的诱导条件不同。由于肝细胞癌患者外周血中BrdUrd/淋巴细胞的比值降低了一半，因此可以排除BrdUrd浓度在诱发肝细胞癌姐妹染色单体交换基础频率中的作用。此外，培养中经Ficoll-Hypaque分离的人单个核细胞(MLC)，但不是猪MLC，其姐妹染色单体交换频率比WBC值高出一倍。因此，无论是细胞分离技术还是中性粒细胞，都不是人类PLC或MLC姐妹染色单体交换频率升高的重要原因。将人红细胞滴定到人PLC中的实验表明，培养液中红细胞的存在以剂量依赖的方式抑制PLC姐妹染色单体交换频率的升高。由于在人PLC和MLC中掺入猪或人红细胞使姐妹染色单体交换频率降低到白细胞的频率，因此推测这些细胞中存在共同的成分和/或功能。对不同RBC成分的分析表明，RBC，特别是RBC幽灵，向培养上清液中释放一种可扩散但不可透析的“校正”因子，能够降低PLC的姐妹染色单体交换频率。抗氧化酶如过氧化氢酶和辣根过氧化物酶不能将人PLC的姐妹染色单体交换频率降低到WBC值。
Sister chromatid exchange (SCE) induction by ultraviolet (UV) light was studied in both human and pig whole blood cultures (WBC) and plasma leukocyte cultures (PLC). No variation in SCE frequency was observed between pig WBC and PLC in control as well as in treated cells. Conversely, SCE frequencies of human PLC were consistently higher than those of WBC in control and UV-exposed cells. Thus, red blood cells (RBCs) do not influence the sensitivity of lymphocytes to UV light exposure, and there must be some different culture condition(s) in the induction of SCEs between human WBC and PLC but not in swine lymphocyte cultures. Since the BrdUrd/lymphocyte ratio of WBC was halved in PLC, the effect of BrdUrd concentration in inducing the SCE baseline frequency of PLC may be ruled out. Also, Ficoll-Hypaque-separated human mononuclear leukocytes in culture (MLC), but not pig MLC, showed a two-fold increase in SCE frequency over WBC values. Thus, neither the cell separation technique nor polymorphonuclear leukocytes had a significant role in the elevated SCE frequency of human PLC or MLC. Experiments where human RBCs were titrated into human PLC showed that the induction of an elevated SCE frequency of PLC was suppressed in a dose-dependent manner by the presence of RBCs in the culture medium. Since the incorporation of pig or human RBCs into human PLC as well as into MLC reduced the SCE frequency to that of WBC, a common component and/or function existing in these cells is suggested. Analysis of different RBC components showed that RBCs, specifically RBC ghosts, release a diffusible but not dialyzable "corrective" factor into culture medium that is able to reduce the SCE frequencies of PLC. Antioxidant enzymes such as catalase and horseradish peroxidase were unable to reduce the SCE frequency of human PLC to WBC values.
布卢姆综合征淋巴细胞中姐妹染色单体交换的细胞融合正常化。
DOI：
10.1126/science.7221565
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Shiraishi,Y;Matsui,S;Sandberg,AA
通讯作者：
Sandberg,AA