Standard operating procedures for serum and plasma collection: early detection research network consensus statement standard operating procedure integration working group.
Standard operating procedures for serum and plasma collection: early detection research network consensus statement standard operating procedure integration working group.
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DOI：
10.1021/pr800545q
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发表时间：
2009-01
影响因子：
4.4
通讯作者：
Brenner DE
中科院分区：
文献类型：
--
作者：
Tuck MK;Chan DW;Chia D;Godwin AK;Grizzle WE;Krueger KE;Rom W;Sanda M;Sorbara L;Stass S;Wang W;Brenner DE
标本采集是临床研究的重要组成部分。来自不同阶段癌症或其他疾病的受试者以及没有疾病的受试者的标本是寻找生物标志物、预测因子或检测的关键工具，这些检测将比目前更早或更容易发现严重疾病。分析方法发展很快。获得高质量的标本，以标准化的方式收集和处理，最大限度地减少潜在的偏差或混淆因素，是实现转化研究“从实验室到床边”目标的关键。在设计临床试验时，必须预先定义标准操作程序，即创建存储库的“方法”。处理或处理样品的微小差异会对分析的可靠性和再现性产生巨大影响，特别是在使用多种方法时。标准操作程序内部工作组（SOPIWG）由来自早期检测研究网络（EDRN）的成员组成，旨在为我们的生物标志物发现和验证工作收集和管理的各种类型的标本制定标准操作程序（sop）。本报告提出了我们对用于生物标志物发现和验证的血清和血浆的收集、处理、处理和储存的标准操作规程的共识。
Specimen collection is an integral component of clinical research. Specimens from subjects with various stages of cancers or other conditions, as well as those without disease, are critical tools in the hunt for biomarkers, predictors, or tests that will detect serious diseases earlier or more readily than currently possible. Analytic methodologies evolve quickly. Access to high-quality specimens, collected and handled in standardized ways that minimize potential bias or confounding factors, is key to the “bench to bedside” aim of translational research. It is essential that standard operating procedures, “the how” of creating the repositories, be defined prospectively when designing clinical trials. Small differences in the processing or handling of a specimen can have dramatic effects in analytical reliability and reproducibility, especially when multiplex methods are used. A representative working group, Standard Operating Procedures Internal Working Group (SOPIWG), comprised of members from across Early Detection Research Network (EDRN) was formed to develop standard operating procedures (SOPs) for various types of specimens collected and managed for our biomarker discovery and validation work. This report presents our consensus on SOPs for the collection, processing, handling, and storage of serum and plasma for biomarker discovery and validation.
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影响因子：
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作者：
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通讯作者：
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