Cryptic diversity within the major trypanosomiasis vector Glossina fuscipes revealed by molecular markers.
Cryptic diversity within the major trypanosomiasis vector Glossina fuscipes revealed by molecular markers.
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DOI：
10.1371/journal.pntd.0001266
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发表时间：
2011-08
影响因子：
3.8
通讯作者：
Donnelly MJ
中科院分区：
文献类型：
--
作者：
Dyer NA;Ravel S;Choi KS;Darby AC;Causse S;Kapitano B;Hall MJ;Steen K;Lutumba P;Madinga J;Torr SJ;Okedi LM;Lehane MJ;Donnelly MJ
采采蝇Glossina fuscipes s.l.非洲锥虫病（HAT）或昏睡病的传播约占90%。三千。主要根据其外生殖器形态上的细微差异，对梭形目亚种进行了描述。在这里，我们描述了一项研究，在这个重要的载体范围内进行，以确定是否从核DNA（微卫星和基因序列信息），线粒体DNA和共生体DNA产生的分子证据支持这些分类群作为离散的分类单位的存在。核核糖体内转录间隔区1（ITS1）为三个亚种提供了支持。然而，核和线粒体序列数据不支持形态亚种G的单系性。F. fuscipes或G. F. quanzensis。相反，最强烈支持的单系群是由来自埃塞俄比亚的苍蝇样本组成的。母系遗传位点（mtDNA和共生体）也表明来自维多利亚湖盆地和坦桑尼亚的一个组的单系性，但这一组不支持核位点，表明这些标记的不同历史。微卫星数据证实了G. fuscipes s.l.，并且对于推导密切相关的种群的相互关系是有用的。我们认为，单靠形态学分类不能对G. fuscipes用于控制目的。埃塞俄比亚的种群计划成为不育昆虫释放方案的对象，但它们的分布非常分散。从方案的角度来看，这可能是积极的，因为它可能反映了有限的迁移到该地区或消极的，如果高度分化也反映在这一人口和释放方案中使用的苍蝇之间的生殖隔离。 褐角舌鱼采采蝇传播了撒哈拉以南非洲地区约90%的非洲人型体线虫病例。以前曾根据形态学理论提出G. fuscipes由三个亚种组成。利用G. fuscipes核，线粒体和共生体的DNA，我们表明，形态亚种不对应以及苍蝇和形态相似的苍蝇之间的遗传差异可能已经出现了不止一次在这个物种的进化。相反，我们发现至少有5个主要的异地分布的G。fuscipes苍蝇。遗传上最独特的蝇类来自埃塞俄比亚，该国计划实施一项不育昆虫释放方案。鉴于采采蝇控制往往利用物种特异性行为，因此迫切需要确定这五个类群的分类地位和范围。此外，鉴于我们只能在物种分布的许多地方进行有限的取样，G.很可能正在等待被发现。
The tsetse fly Glossina fuscipes s.l. is responsible for the transmission of approximately 90% of cases of human African trypanosomiasis (HAT) or sleeping sickness. Three G. fuscipes subspecies have been described, primarily based upon subtle differences in the morphology of their genitalia. Here we describe a study conducted across the range of this important vector to determine whether molecular evidence generated from nuclear DNA (microsatellites and gene sequence information), mitochondrial DNA and symbiont DNA support the existence of these taxa as discrete taxonomic units. The nuclear ribosomal Internal transcribed spacer 1 (ITS1) provided support for the three subspecies. However nuclear and mitochondrial sequence data did not support the monophyly of the morphological subspecies G. f. fuscipes or G. f. quanzensis. Instead, the most strongly supported monophyletic group was comprised of flies sampled from Ethiopia. Maternally inherited loci (mtDNA and symbiont) also suggested monophyly of a group from Lake Victoria basin and Tanzania, but this group was not supported by nuclear loci, suggesting different histories of these markers. Microsatellite data confirmed strong structuring across the range of G. fuscipes s.l., and was useful for deriving the interrelationship of closely related populations. We propose that the morphological classification alone is not used to classify populations of G. fuscipes for control purposes. The Ethiopian population, which is scheduled to be the target of a sterile insect release (SIT) programme, was notably discrete. From a programmatic perspective this may be both positive, given that it may reflect limited migration into the area or negative if the high levels of differentiation are also reflected in reproductive isolation between this population and the flies to be used in the release programme. Glossina fuscipes s.l. tsetse flies are responsible for transmission of approximately 90% of the cases of Human African Typanosomiasis in Sub Saharan Africa. It was previously proposed on the basis of morphology that G. fuscipes is composed of three sub-species. Using genetic evidence from G. fuscipes nuclear, mitochondrial and symbiont DNA, we show that the morphological subspecies do not correspond well to genetic differences between the flies and morphologically similar flies may have arisen more than once in the evolution of this species. Instead, we found at least 5 main allopatrically distributed groups of G. fuscipes flies. The most genetically distinct group of flies originated from Ethiopia, where a sterile insect release programme is planned. Given that tsetse control often exploits species-specific behaviours there is a pressing need to establish the taxonomic status and ranges of these five groups. Moreover given that we were only able to perform limited sampling in many parts of the species distribution further groups within G. fuscipes are likely to be awaiting discovery.
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DOI：
10.1111/j.1096-0031.1996.tb00196.x
发表时间：
1994-09-01
期刊：
CLADISTICS-THE INTERNATIONAL JOURNAL OF THE WILLI HENNIG SOCIETY
影响因子：
--
作者：
FARRIS, JS;KALLERSJO, M;BULT, C
通讯作者：
BULT, C
影响因子：
30.8
作者：
Akman, L;Yamashita, A;Aksoy, S
通讯作者：
Aksoy, S
DOI：
10.1111/j.2517-6161.1977.tb01600.x
发表时间：
1977-01-01
期刊：
JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-METHODOLOGICAL
影响因子：
--
作者：
DEMPSTER, AP;LAIRD, NM;RUBIN, DB
通讯作者：
RUBIN, DB
影响因子：
10.7
作者：
Chapuis, Marie-Pierre;Estoup, Arnaud
通讯作者：
Estoup, Arnaud
影响因子：
4.1
作者：
Farrell, BD
通讯作者：
Farrell, BD