Effect of adenosine on the formation of prostacyclin in the rabbit isolated heart
Effect of adenosine on the formation of prostacyclin in the rabbit isolated heart
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腺苷对兔离体心脏前列环素形成的影响
DOI：
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发表时间：
1988
影响因子：
7.3
通讯作者：
Å. Wennmalm
中科院分区：
文献类型：
--
作者：
E. Karwatowska;G. Ciabattoni;Å. Wennmalm
1观察腺苷对心脏前列环素（PGI_2）生物合成的影响。根据Langendorff在控制压力（有或没有茶碱）或控制流量下灌注兔心脏。使用高度特异性放射免疫测定法测定基础条件下和腺苷输注期间冠状动脉流出液中6-酮-前列腺素F1 α（6-酮-前列腺素F1 α，PGI 2的代谢产物）的含量。2在其他实验中，将兔主动脉环与或不与腺苷一起孵育，并如上所述分析6-keto-PGF 1 α的产生。3在控制压力下灌注的心脏中给予腺苷（10 μm）可使冠状动脉流量增加38%。流出液中6-keto-PGF 1 α的峰浓度超过对照组的177%（P < 0.01），6-keto-PGF 1 α的总流出量超过对照组的179%（P < 0.001）。茶碱（50 μm）可分别降低腺苷的这些作用23%、43%和51%，而不影响心脏对腺苷的摄取。4当腺苷（1-10 μm）以受控流量灌注心脏时，观察到灌注压呈剂量依赖性降低，分别降低27%和44%。同时，6-keto-PGF 1 α外排的增加显著较低（分别为49%（P < 0.05）和43%（NS））。在不存在腺苷的情况下诱导的灌注压的类似降低分别使6-keto-PGF 1 α的外排减少15%（P < 0.01）和32%（P < 0.001）。5与对照相比，向兔主动脉环孵育物中加入腺苷（1-10 μm）对孵育培养基中6-酮-PGF 1 α的浓度无显著影响。6我们得出结论，腺苷刺激兔心脏PGI 2的形成，主要是通过与核苷的血管扩张作用有关的作用。
1 The effect of adenosine on cardiac biosynthesis of prostacyclin (PGI2) was investigated. Rabbit hearts were perfused according to Langendorff at controlled pressure (with or without theophylline), or at controlled flow. The content of 6‐keto‐prostaglandinlflt (6‐keto‐PGF1α, metabolite of PGI2) in the coronary effluent under basal conditions and during infusion of adenosine was determined using a highly specific radioimmunoassay. 2 In other experiments, rings of rabbit aorta were incubated with or without adenosine and the production of 6‐keto‐PGF1α was analysed as above. 3 Administration of adenosine (10 μm) to hearts perfused at controlled pressure increased the coronary flow by up to 38%. The peak concentration of 6‐keto‐PGF1α in the effluent exceeded the control by 177% (P < 0.01), and the total efflux of 6‐keto‐PGF1α exceeded the control by 179% (P < 0.001). Theophylline (50 μm) reduced these effects of adenosine by 23%, 43% and 51%, respectively, without influencing the uptake of adenosine into the heart. 4 When adenosine (1–10 μm) was administered to hearts perfused at controlled flow, a dose‐dependent decrease in the perfusion pressure, by 27% and 44% respectively, was observed. In parallel, the resulting increase in 6‐keto‐PGF1α efflux was considerably lower (49% (P < 0.05) and 43% (NS), respectively). A similar decrease in perfusion pressure induced in the absence of adenosine decreased the efflux of 6‐keto‐PGF1α, by 15% (P < 0.01) and 32% (P < 0.001), respectively. 5 Addition of adenosine (1–10 μm) to incubates of rabbit aortic rings did not significantly affect the concentration of 6‐keto‐PGF1α in the incubation medium in comparison with control. 6 We conclude that adenosine stimulates rabbit heart PGI2 formation, mainly by an action related to the vasodilator effect of the nucleoside.
影响因子：
56.9
作者：
FRANGOS, JA;ESKIN, SG;IVES, CL
通讯作者：
IVES, CL