The WOX family transcriptional regulator SlLAM1 controls compound leaf and floral organ development in Solanum lycopersicum.
The WOX family transcriptional regulator SlLAM1 controls compound leaf and floral organ development in Solanum lycopersicum.
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WOX家族转录调节因子SlLAM1控制番茄复合叶和花器官的发育
DOI：
10.1093/jxb/eraa574
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发表时间：
2021-02-27
影响因子：
6.9
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Wang C;Zhao B;He L;Zhou S;Liu Y;Zhao W;Guo S;Wang R;Bai Q;Li Y;Wang D;Wu Q;Yang Y;Liu Y;Tadege M;Chen J
LAM 1转录因子调节番茄复叶中初级小叶的扩展，并且还影响花器官发育、果实大小和次级小叶的起始。植物特异性WOX家族转录因子在胚胎发生和侧生器官发育过程中发挥着重要作用。WOX 1转录因子，属于现代进化枝的WOX家族，是已知的调节生长的叶片特异性地在mediolateral轴;然而，WOX 1在复叶发育中的作用仍然未知。对番茄WOX基因家族的系统发育分析表明，该家族共有10个成员，分别代表了现代、中间和古老的分支。利用系统发育分析和反向遗传方法，在这项研究中，我们确定了SlLAM 1的现代分支，并检查其功能和组织特异性表达模式。我们发现，通过CRISPR/Cas9介导的基因组编辑敲除SlLAM 1导致叶片变窄，次级小叶数量减少。过量表达番茄SlLAM 1可以挽救烟草lam 1突变体的缺陷。解剖学和转录组学分析表明，花器官发育，果实大小，次级小叶起始，叶的复杂性改变，由于SlLAM 1的功能丧失。这些研究结果表明，番茄SlLAM 1起着重要的作用，在调节次级小叶的启动，除了其保守的功能，在叶片扩展。
The LAM1 transcription factor regulates expansion of primary leaflets in the compound leaves of tomato, and also affects floral organ development, fruit size, and initiation of secondary leaflets. Plant-specific WOX family transcription factors play important roles ranging from embryogenesis to lateral organ development. The WOX1 transcription factors, which belong to the modern clade of the WOX family, are known to regulate outgrowth of the leaf blade specifically in the mediolateral axis; however, the role of WOX1 in compound leaf development remains unknown. Phylogenetic analysis of the whole WOX family in tomato (Solanum lycopersicum) indicates that there are 10 members that represent the modern, intermediate, and ancient clades. Using phylogenetic analysis and a reverse genetic approach, in this study we identified SlLAM1 in the modern clade and examined its function and tissue-specific expression pattern. We found that knocking out SlLAM1 via CRISPR/Cas9-mediated genome editing led to narrow leaves and a reduced number of secondary leaflets. Overexpression of tomato SlLAM1 could rescue the defects of the tobacco lam1 mutant. Anatomical and transcriptomic analyses demonstrated that floral organ development, fruit size, secondary leaflet initiation, and leaf complexity were altered due to loss-of-function of SlLAM1. These findings demonstrate that tomato SlLAM1 plays an important role in the regulation of secondary leaflet initiation, in addition to its conserved function in blade expansion.
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影响因子：
9.2
作者：
Eshed, Y;Baum, SF;Bowman, JL
通讯作者：
Bowman, JL
影响因子：
3
作者：
FEDER, N;OBRIEN, TP
通讯作者：
OBRIEN, TP
影响因子：
27.5
作者：
Dolzblasz, Alicja;Nardmann, Judith;Laux, Thomas
通讯作者：
Laux, Thomas
影响因子：
1.1
作者：
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通讯作者：
Sinha, Neelima R.
影响因子：
64.8
作者：
Kerstetter, RA;Bollman, K;Poethig, RS
通讯作者：
Poethig, RS