A novel phosphotransferase system of Streptococcus mutans is responsible for transport of carbohydrates with α-1,3 linkage.
A novel phosphotransferase system of Streptococcus mutans is responsible for transport of carbohydrates with α-1,3 linkage.
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DOI：
10.1111/omi.12009
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发表时间：
2013-04
影响因子：
3.7
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Ajdic D;Chen Z
变形链球菌中最常见的碳水化合物转运系统是磷酸烯醇丙酮酸(PEP)-糖磷酸转移酶（PTS）系统。我们之前发现变形链球菌UA159中存在14个PTSs。一些研究表明，与浮游生物相比，生长在生物膜中的微生物表达不同的基因。在这项研究中，我们发现变形链球菌的一个PTS在蔗糖生长的生物膜中表达。此外，相同的PTS还负责双糖黑糖（3-O-α-D-glucopyranosyl-D-glucose）的运输和代谢。此外，研究结果表明，所研究的PTS可能参与了变形链球菌葡萄糖基转移酶B （GtfB）和葡萄糖基转移酶C （GtfC）合成的碳水化合物的转运和代谢。据我们所知，这是第一个显示具有α-1,3连锁的双糖（可能是细胞外低聚糖）的PTS转运的报告。
The most common type of carbohydrate-transport system in Streptococcus mutans is the phosphoenolpyruvate (PEP)-sugar phosphotransferase system (PTS). We previously showed that fourteen PTSs exist in S. mutans UA159. Several studies have shown that microorganisms growing in biofilms express different genes as compared to their planktonic counterparts. In this study, we showed that one PTS of S. mutans was expressed in sucrose-grown biofilms. Furthermore, the same PTS was also responsible for the transport and metabolism of disaccharide nigerose (3-O-α-D-glucopyranosyl-D-glucose). Additionally, the results indicate that the studied PTS might be involved in the transport and metabolism of carbohydrates synthesized by glucosyltransferase B (GtfB) and glucosyltransferase C (GtfC) of S. mutans. To our knowledge, this is the first report that shows PTS transport of a disaccharide (and possibly extracellular oligosaccharides) with α-1,3 linkage.
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影响因子：
3.1
作者：
HANADA, N;KURAMITSU, HK
通讯作者：
KURAMITSU, HK
影响因子：
2.7
作者：
EBISU, S;KATO, K;MISAKI, A
通讯作者：
MISAKI, A
影响因子：
7.6
作者：
BRADSHAW, DJ;MCKEE, AS;MARSH, PD
通讯作者：
MARSH, PD
影响因子：
7.6
作者：
HAMADA, S;KOGA, T;OOSHIMA, T
通讯作者：
OOSHIMA, T
影响因子：
3.2
作者：
Ajdic, Dragana;Pham, Vi T. T.
通讯作者：
Pham, Vi T. T.