Whole-cell fluorescent biosensors for bioavailability and biodegradation of polychlorinated biphenyls.

Whole-cell fluorescent biosensors for bioavailability and biodegradation of polychlorinated biphenyls.
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DOI:
10.3390/s100201377
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发表时间:
2010
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Dowling DN
Dowling DN
中科院分区:
其他
文献类型:
--
作者:
Liu X;Germaine KJ;Ryan D;Dowling DN

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全细胞微生物生物传感器是环境监测中使用的最新分子工具之一。此类生物传感器是通过将 lux、gfp 或 lacZ 等报告基因与响应启动子融合而构建的。关于生物传感器的应用已有许多报道,特别是它们在测定污染物毒性和生物利用度方面的应用。本文回顾了构建用于污染物监测的全细胞微生物生物传感器的基本概念,并描述了两种此类生物传感器在检测多氯联苯(PCB)的生物利用度和生物降解性方面的应用。
Whole-cell microbial biosensors are one of the newest molecular tools used in environmental monitoring. Such biosensors are constructed through fusing a reporter gene such as lux, gfp or lacZ, to a responsive promoter. There have been many reports of the applications of biosensors, particularly their use in assaying pollutant toxicity and bioavailability. This paper reviews the basic concepts behind the construction of whole-cell microbial biosensors for pollutant monitoring, and describes the applications of two such biosensors for detecting the bioavailability and biodegradation of Polychlorinated Biphenyls (PCBs).
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