Molecular epidemiology of porcine epidemic diarrhea virus in China.
Molecular epidemiology of porcine epidemic diarrhea virus in China.
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中国猪流行性腹泻病毒的分子流行病学
DOI：
10.1007/s00705-010-0720-2
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发表时间：
2010-09
影响因子：
2.7
通讯作者：
Feng L
中科院分区：
文献类型：
--
作者：
Chen J;Wang C;Shi H;Qiu H;Liu S;Chen X;Zhang Z;Feng L
自2006年初以来，猪流行性腹泻病毒（PEDV）在免疫猪群中再次出现。开放阅读框3（ORF 3）是PEDV基因组中唯一的辅助基因。对12株PEDV野毒株和1株疫苗株的ORF 3基因进行了全序列测定。中国PEDV野毒株（CH/GSJIII/07除外）的ORF 3基因含有一个672或675个核苷酸（nt）的ORF，编码一个223或224 aa长的多肽。然而，CV 777疫苗株和CH/GSJIII/07含有276-nt ORF，因为在nt 245-293处缺失49-nt。根据ORF 3基因的系统发育关系将我国PEDV野毒株和参考毒株分为3个类群。中国PEDV野毒株（CH/GSJIII/07除外）与韩国株的亲缘关系较近，与疫苗株的遗传差异较大。然而，CH/GSJIII/07与两种疫苗株的亲缘关系较近，表明其可能是由活疫苗株进化而来。用巢式RT-PCR方法可以区分中国PEDV野毒株（CH/GSJIII/07除外）和PEDV疫苗株。
Since early 2006, porcine epidemic diarrhea virus (PEDV) has been reemerging in immunized swine herds. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. The entire ORF3 genes of 12 PEDV field strains and one vaccine strain were sequenced. The ORF3 genes of Chinese PEDV field strains (excluding CH/GSJIII/07) contain a single 672- or 675-nucleotide (nt) ORF, which encodes a 223- or 224-aa-long peptide. However, the CV777 vaccine strain and CH/GSJIII/07 contain a 276-nt ORF because of a 49-nt deletion at nt 245–293. The Chinese PEDV field strains and PEDV reference strains are divided into three groups based on the phylogenetic relationship of their ORF3 genes. Chinese PEDV field strains (excluding CH/GSJIII/07) have a close phylogenetic relationship to Korean strains and are genetically different from the PEDV vaccine strains. However, CH/GSJIII/07 has a close phylogenetic relationship to two vaccine strains, suggesting that it might have evolved from a live vaccine strain. Chinese PEDV field strains (excluding CH/GSJIII/07) can be differentiated from PEDV vaccine strains by a nested RT-PCR method.
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影响因子：
3.7
作者：
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通讯作者：
DEGROOT, RJ
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作者：
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