Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot.
Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot.
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DOI：
10.1016/j.clim.2004.07.003
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发表时间：
2004-11
期刊：
影响因子：
--
通讯作者：
Chen WF
中科院分区：
文献类型：
--
作者：
Wang YD;Li Y;Xu GB;Dong XY;Yang XA;Feng ZR;Tian C;Chen WF
产生来自严重急性呼吸综合征（SARS）冠状病毒（SARA-CoV）的S蛋白的重组片段，并将其用于与商业SARS ELISA方法进行比较的蛋白质印迹（WB）测定。在85%的确诊SARS病例（n = 20）中，基于S2重组片段的WB呈阳性，这与使用热灭活SARS-CoV的商业ELISA相当。用其他4个重组片段在SARS确诊病例中的WB检出率较低（S1- 75%，S1-N-25%，S1-C-55%）。健康对照组（n = 60）血清的ELISA检测结果为2例弱阳性，其余均为阴性，而S2蛋白WB检测结果显示20例有SARS接触史的对照组中有3例阳性，40例无SARS接触史的对照组中无阳性。当评价来自具有SARS样症状的个体（n = 10）的per-2003血清时，ELISA和S2 WB之间出现差异（ELISA-100%阳性，S2 WB-30%阳性）。这些数据表明，S2 WB检测可能是特别有用的ELISA阴性SARS病例和一些ELISA阳性非SARS病例。
Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases (n = 20), the S2 recombinant fragment based WB was positive and this was comparable to the commercial ELISA using heat killed SARS-CoV. WB using the other four recombinant fragments in confirmed SARS cases generated lower rates of detection (S1—75%, S1-N—25%, S1-C—55%). Evaluation of sera from healthy controls (n = 60) resulted in two weakly positive ELISA results with the remainder being negative while the S2 protein WB demonstrated three positive results from the 20 controls with a history of SARS contact and no positive results in 40 noncontact controls. A discrepancy between the ELISA and S2 WB arose when evaluating per-2003 sera from individuals (n = 10) with SARS-like symptoms (ELISA—100% positive, S2 WB—30% positive). These data suggest that the S2 WB assay may be particularly useful in ELISA-negative SARS cases and in some ELISA-positive non-SARS cases.
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影响因子：
3.7
作者：
Stewart JN;Mounir S;Talbot PJ
通讯作者：
Talbot PJ
DOI：
10.1016/j.bbrc.2003.11.180
发表时间：
2004-01-23
影响因子：
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作者：
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作者：
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BUCHMEIER, MJ
影响因子：
11.8
作者：
Vabret, A;Mourez, T;Freymuth, F
通讯作者：
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影响因子：
5.4
作者：
Babcock, GJ;Esshaki, DJ;Ambrosino, DM
通讯作者：
Ambrosino, DM