Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis
Transcriptome analysis reveals in vitro-cultured regeneration bulbs as a promising source for targeted Fritillaria cirrhosa steroidal alkaloid biosynthesis
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转录组分析揭示体外培养的再生鳞茎是有前景的川贝母甾体生物碱生物合成来源
DOI：
10.1007/s13205-018-1218-y
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发表时间：
2018-03
期刊：
影响因子：
2.8
通讯作者：
Li Jian
中科院分区：
文献类型：
--
作者：
Zhao Qi;Li Rui;Zhang Yang;Huang Kejia;Wang Wenguo;Li Jian
The bulbs ofFritillaria cirrhosais wildly used in traditional Chinese medicine to treat lung-related disease, which has recently been found to have antitussive, anti-inflammatory, antihypertensive and anti-tumor activity. Steroidal alkaloids are the major effective ingredients ofF. cirrhosa. In the current study, we demonstrated an efficient strategy forF. cirrhosabulb regeneration in vitro by cytokinin/auxin induction. Our data showed that the regenerated bulbs accumulated higher alkaloid content that the wild ones. We further performed RNA-seq and bioinformatics analysis to study the gene expression profile, especially those related to alkaloids biosynthesis. KEGG pathway annotation identified genes related to "Metabolic pathways" were the most abundant (2644, 26.0%), followed by those for "Biosynthesis of secondary metabolites" (1319, 13.0%) among the 113,865 unigenes identified. Further analysis suggested MEP pathway, other than MVA pathway, might be the major route for steroidal alkaloid biosynthesis ofF. cirrhosa, as all the key genes in this pathway were found to be unregulated in our study. We also showed that accumulation of different phytochemicals was linked to plant hormone addition. Our current study demonstrated that in vitro cultivation is a promising strategy for mass production ofF. cirrhosasteroidal alkaloids for pharmacological industry.
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DOI：
10.1515/znc-1982-1-209
发表时间：
1982-02
期刊：
Zeitschrift für Naturforschung C
影响因子：
--
作者：
T. Bach;H. Lichtenthaler
通讯作者：
T. Bach;H. Lichtenthaler
影响因子：
7.2
作者：
Zhang, Hongtao;Hedhili, Sabah;Memelink, Johan
通讯作者：
Memelink, Johan
DOI：
10.1007/978-94-017-9223-3_14
发表时间：
2014
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
--
作者：
So-Young Park;K. Paek
通讯作者：
So-Young Park;K. Paek
影响因子：
--
作者：
Sanjay Pant;E. Chiprout;D. Blaauw
通讯作者：
Sanjay Pant;E. Chiprout;D. Blaauw
影响因子：
4.3
作者：
Pal, Tarun;Malhotra, Nikhil;Chauhan, Rajinder Singh
通讯作者：
Chauhan, Rajinder Singh