Microscale tissue engineering of liver lobule models: advancements and applications.
Microscale tissue engineering of liver lobule models: advancements and applications.
复制标题
DOI：
10.3389/fbioe.2023.1303053
复制
发表时间：
2023
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
肝脏是人体维持内部平衡的主要器官，由许多六角形肝小叶组成，每个肝小叶都具有统一的结构框架。这些肝小叶是肝脏的基本结构和功能单位，由中央静脉、肝板、肝窦和微小胆管组成。同时，在肝小叶内，肝细胞的不同区域执行不同的功能。体外构建肝小叶模型，忠实地复制其结构和功能，对于肝脏发育和疾病的研究具有至关重要的意义。目前，构建肝小叶模型的两种主要技术在该领域占据主导地位：3D生物打印和微流体技术。 3D生物打印能够实现细胞和生物材料的精确沉积，而微流控技术则有助于将细胞或其他培养材料定向运输到指定位置，通过微泵控制有效管理培养基的输入和输出，从而实现肝小叶的动态模拟。在这篇综合综述中，我们概述了最近研究人员在构建肝小叶模型时所采用的生物材料、细胞和制造方法。我们的目标是探索密切模拟肝小叶真实结构和功能的策略和技术，为肝病研究、药物筛选、药物毒性评估和细胞替代疗法提供宝贵的见解。
The liver, as the body’s primary organ for maintaining internal balance, is composed of numerous hexagonal liver lobules, each sharing a uniform architectural framework. These liver lobules serve as the basic structural and functional units of the liver, comprised of central veins, hepatic plates, hepatic sinusoids, and minute bile ducts. Meanwhile, within liver lobules, distinct regions of hepatocytes carry out diverse functions. The in vitro construction of liver lobule models, faithfully replicating their structure and function, holds paramount significance for research in liver development and diseases. Presently, two primary technologies for constructing liver lobule models dominate the field: 3D bioprinting and microfluidic techniques. 3D bioprinting enables precise deposition of cells and biomaterials, while microfluidics facilitates targeted transport of cells or other culture materials to specified locations, effectively managing culture media input and output through micro-pump control, enabling dynamic simulations of liver lobules. In this comprehensive review, we provide an overview of the biomaterials, cells, and manufacturing methods employed by recent researchers in constructing liver lobule models. Our aim is to explore strategies and technologies that closely emulate the authentic structure and function of liver lobules, offering invaluable insights for research into liver diseases, drug screening, drug toxicity assessment, and cell replacement therapy.
影响因子：
5.6
作者：
Abdellatef SA;Ohi A;Nabatame T;Taniguchi A
通讯作者：
Taniguchi A
影响因子：
3.9
作者：
Macdonald, N. P.;Menachery, A.;Reboud, J.;Cooper, J. M.
通讯作者：
Cooper, J. M.
影响因子：
25.7
作者：
Utoh R;Komori J;Kuge H;Tatsumi K;Yamada M;Hirohashi S;Tsutsumi M;Amanuma T;Yoshioka A;Nakajima Y;Wake K;Okano T;Lagasse E;Ohashi K
通讯作者：
Ohashi K