The effect of physical and chemical cues on hepatocellular function and morphology.
The effect of physical and chemical cues on hepatocellular function and morphology.
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DOI：
10.3390/ijms15034299
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发表时间：
2014-03-11
影响因子：
5.6
通讯作者：
Taniguchi A
中科院分区：
文献类型：
--
作者：
Abdellatef SA;Ohi A;Nabatame T;Taniguchi A
细胞外基质(ECM)的物理地貌特征和/或化学刺激提供了操纵细胞功能的基本线索。从物理角度来看，轮廓纳米结构对于细胞行为和细胞功能是非常重要的。从化学角度来看，已知含有RGD序列的ECM蛋白可以改变细胞功能。在这项研究中，研究了综合物理和化学线索对肝细胞系(HepG2)的影响。为了模拟ECM提供的物理线索，制备了具有特定尺寸和几何特征(纳米级宽90 nm，间距150 nm)的非晶态二氧化钛纳米级。为了模拟ECM提供的化学线索，通过固定化RGD基序对二氧化钛无机薄膜进行了修饰。研究了不同信号组合对肝细胞株形态和功能的影响，包括细胞排列和不同功能蛋白的表达。纳米粒子和表面修饰与RGD的结合诱导了细胞的排列和功能蛋白的表达，表明物理和化学线索是优化肝细胞功能的重要因素。
Physical topographical features and/or chemical stimuli to the extracellular matrix (ECM) provide essential cues that manipulate cell functions. From the physical point of view, contoured nanostructures are very important for cell behavior in general, and for cellular functions. From the chemical point of view, ECM proteins containing an RGD sequence are known to alter cell functions. In this study, the influence of integrated physical and chemical cues on a liver cell line (HepG2) was investigated. To mimic the physical cues provided by the ECM, amorphous TiO2 nanogratings with specific dimensional and geometrical characteristics (nanogratings 90 nm wide and 150 nm apart) were fabricated. To mimic the chemical cues provided by the ECM, the TiO2 inorganic film was modified by immobilization of the RGD motif. The hepatic cell line morphological and functional changes induced by simultaneously combining these diversified cues were investigated, including cellular alignment and the expression of different functional proteins. The combination of nanopatterns and surface modification with RGD induced cellular alignment and expression of functional proteins, indicating that physical and chemical cues are important factors for optimizing hepatocyte function.
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DOI：
10.1083/jcb.20.2.271
发表时间：
1964-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
HAMASHIMA Y;HARTER JG;COONS AH
通讯作者：
COONS AH
影响因子：
6.9
作者：
Heino, J
通讯作者：
Heino, J
影响因子：
19
作者：
Lukashev, ME;Werb, Z
通讯作者：
Werb, Z
影响因子：
3.3
作者：
Herrema, Hilde;Czajkowska, Dorninika;van Ijzendoorn, Sven C. D.
通讯作者：
van Ijzendoorn, Sven C. D.
影响因子：
--
作者：
Park, Kyung Min;Park, Ki Dong
通讯作者：
Park, Ki Dong