Fabrication and Characterization of PCL/PLGA Coaxial and Bilayer Fibrous Scaffolds for Tissue Engineering.
Fabrication and Characterization of PCL/PLGA Coaxial and Bilayer Fibrous Scaffolds for Tissue Engineering.
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DOI：
10.3390/ma14216295
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发表时间：
2021-10-22
期刊：
影响因子：
--
通讯作者：
Sefat F
中科院分区：
文献类型：
--
作者：
Bazgir M;Zhang W;Zhang X;Elies J;Saeinasab M;Coates P;Youseffi M;Sefat F
静电纺丝是一种创新的纤维技术，旨在设计和制造适用于包括血管移植在内的广泛组织工程（TE）应用的膜，这是本研究工作的主要目标。本研究涉及双层结构的制造和表征，该双层结构由聚己内酯制成的电纺片（PCL，内层）和聚乳酸-乙醇酸共聚物（PLGA）制成的外层组成，并成功生产出具有微米纤维结构的同轴多孔支架。通过评估膜在经历降解过程时的形态结构/物理特性和结构完整性，评估膜是否适合预期的生物医学应用。使用扫描电子显微镜 (SEM) 评估静电纺支架结构形态的变化，例如纤维直径、孔径 (μm) 以及使用 Image J 软件测量的支架表面的孔隙率。在室温下12周的降解过程中，除了60分钟的支架外，大多数支架的降解率都表现出相似的趋势。同轴支架的质量损失显着低于双层 PCL/PLGA 支架，分别为 1.348% 和 18.3%。测量了纤维膜的机械性能，与双层支架相比，同轴支架表现出更大的拉伸强度和断裂伸长率 (%)。根据本研究获得的结果，可以得出结论，用同轴针制成的支架更适合组织工程应用，因为与基本静电纺丝工艺相比，所得聚合物膜的质量和功能得到了改善。然而，虽然制造血管移植物是这项研究工作的主要目的，但生物学数据不会出现在本文中。
Electrospinning is an innovative new fibre technology that aims to design and fabricate membranes suitable for a wide range of tissue engineering (TE) applications including vascular grafts, which is the main objective of this research work. This study dealt with fabricating and characterising bilayer structures comprised of an electrospun sheet made of polycaprolactone (PCL, inner layer) and an outer layer made of poly lactic-co-glycolic acid (PLGA) and a coaxial porous scaffold with a micrometre fibre structure was successfully produced. The membranes’ propriety for intended biomedical applications was assessed by evaluating their morphological structure/physical properties and structural integrity when they underwent the degradation process. A scanning electron microscope (SEM) was used to assess changes in the electrospun scaffolds’ structural morphology such as in their fibre diameter, pore size (μm) and the porosity of the scaffold surface which was measured with Image J software. During the 12-week degradation process at room temperature, most of the scaffolds showed a similar trend in their degradation rate except the 60 min scaffolds. The coaxial scaffold had significantly less mass loss than the bilayer PCL/PLGA scaffold with 1.348% and 18.3%, respectively. The mechanical properties of the fibrous membranes were measured and the coaxial scaffolds showed greater tensile strength and elongation at break (%) compared to the bilayer scaffolds. According to the results obtained in this study, it can be concluded that a scaffold made with a coaxial needle is more suitable for tissue engineering applications due to the improved quality and functionality of the resulting polymeric membrane compared to the basic electrospinning process. However, whilst fabricating a vascular graft is the main aim of this research work, the biological data will not present in this paper.
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DOI：
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影响因子：
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DOI：
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发表时间：
2007-01-01
影响因子：
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