Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases.
Lateralized gustatory behavior of C. elegans is controlled by specific receptor-type guanylyl cyclases.
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DOI：
10.1016/j.cub.2009.05.043
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发表时间：
2009-06-23
期刊：
影响因子：
--
通讯作者：
Hobert O
中科院分区：
文献类型：
--
作者：
Ortiz CO;Faumont S;Takayama J;Ahmed HK;Goldsmith AD;Pocock R;McCormick KE;Kunimoto H;Iino Y;Lockery S;Hobert O
尽管功能性偏侧化是许多神经系统的主要特征，但人们对偏侧化神经功能与偏侧化基因活性之间的联系知之甚少。线虫C. elegans，ASEL和ASER，作为一个模型来研究功能偏侧化的遗传基础，因为这对传感器的数量在左/右不对称的方式化学品。ASE神经元的功能偏侧化程度和负责ASEL/R的左/右不对称活性的基因尚不清楚。我们在这里表明，一个大的面板的盐离子感测在左/右不对称的方式，表现出的行为分析，神经活动的成像与遗传编码的钙传感器和遗传操作，改变ASEL或ASER的命运。我们发现，偏侧盐反应允许蠕虫区分不同的盐线索。为了鉴定可能参与感知盐离子和/或传递这种感觉信息的分子，我们检测了8种不同受体类型跨膜鸟苷酸环化酶突变的动物的趋化行为（由gcy基因编码），其在ASEL（gcy-6、gcy-7、gcy-14）、ASER（gcy-1、gcy-4、gcy-5、gcy-22）或ASEL和ASER（gcy-19）中表达。破坏ASER表达的gcy基因，gcy-22，导致广泛的趋化缺陷，几乎所有的盐感ASER，以及左/右不对称感测的氨基酸。与此相反，其他gcy基因的破坏导致高度盐离子特异性化学感受缺陷。此外，我们表明，不仅环化酶结构域，而且细胞外结构域的GCY蛋白是重要的，他们的活动在盐的感觉。我们的研究结果拓宽了我们对神经功能偏侧性的理解，提供了对这种偏侧性如何被分子编码的见解，并揭示了参与味觉信号转导的信号分子的异常多样性。
Even though functional lateralization is a predominant feature of many nervous systems, it is poorly understood how lateralized neural function is linked to lateralized gene activity. A bilaterally symmetric pair of gustatory neurons in the nematode C. elegans, ASEL and ASER, serves as a model to study the genetic basis of functional lateralization as this pair senses a number of chemicals in a left/right asymmetric manner. The extent of functional lateralization of the ASE neurons and genes responsible for the left/right asymmetric activity of ASEL/R are unknown. We show here that a large panel of salt ions is sensed in a left/right asymmetric manner, as demonstrated by behavioral assays, imaging of neural activity with a genetically encoded calcium sensor and by genetic manipulations that alter the fate of either ASEL or ASER. We show that lateralized salt responses allow the worm to discriminate between distinct salt cues. To identify molecules that may be involved in sensing salt ions and/or transmitting such sensory information, we examined the chemotaxis behavior of animals harboring mutations in eight different receptor-type, transmembrane guanylyl cyclases (encoded by gcy genes), which are expressed in either ASEL (gcy-6, gcy-7, gcy-14), ASER (gcy-1, gcy-4, gcy-5, gcy-22) or ASEL and ASER (gcy-19). Disruption of a ASER-expressed gcy gene, gcy-22, resulted in a broad chemotaxis defect to nearly all salts sensed by ASER, as well as to a left/right-asymmetrically sensed amino acid. In contrast, disruption of other gcy genes resulted in highly salt ion-specific chemosensory defects. Furthermore, we show that not only the cyclase domain, but also the extracellular domain of GCY proteins is important for their activity in salt sensation. Our findings broaden our understanding of lateralities in neural function, provide insights into how this laterality is molecularly encoded and reveal an unusually diverse spectrum of signaling molecules involved in gustatory signal transduction.
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影响因子：
64.8
作者：
Suzuki, Hiroshi;Thiele, Tod R.;Faumont, Serge;Ezcurra, Marina;Lockery, Shawn R.;Schafer, William R.
通讯作者：
Schafer, William R.
DOI：
10.1073/pnas.0610877104
发表时间：
2007-02-13
影响因子：
11.1
作者：
Chelur, Dattananda S.;Chalfie, Martin
通讯作者：
Chalfie, Martin
影响因子：
3.7
作者：
Frokjaer-Jensen, Christian;Ailion, Michael;Lockery, Shawn R.
通讯作者：
Lockery, Shawn R.
影响因子：
9.2
作者：
Law, E;Nuttley, WM;van der Kooy, D
通讯作者：
van der Kooy, D
影响因子：
64.8
作者：
van den Akker, F;Zhang, XL;Yee, VC
通讯作者：
Yee, VC