Quaternary structure of the Mr 46,000 mannose 6-phosphate specific receptor: effect of ligand, pH, and receptor concentration on the equilibrium between dimeric and tetrameric receptor forms.
Quaternary structure of the Mr 46,000 mannose 6-phosphate specific receptor: effect of ligand, pH, and receptor concentration on the equilibrium between dimeric and tetrameric receptor forms.
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Mr 46,000 甘露糖 6-磷酸特异性受体的四级结构：配体、pH 和受体浓度对二聚体和四聚体受体形式之间平衡的影响。
作者：
A. Waheed;A. Hille;U. Junghans;K. von Figura
The Mr 46,000 mannose 6-phosphate specific receptor exists in solution as a mixture of noncovalently associated dimeric and tetrameric forms. The two quaternary forms were separated by sucrose density centrifugation, and their composition was assessed by cross-linking with bifunctional reagents followed by SDS-polyacrylamide gel electrophoresis. The dependence of equilibrium between the dimeric and tetrameric forms on pH, receptor concentration, and presence of mannose 6-phosphate was studied. The formation of tetrameric forms is favored by pH values around 7, high receptor concentration, and presence of mannose 6-phosphate ligand. Tetrameric forms bind stronger at pH 7 to phosphomannan-Sepharose 4B than dimeric forms. Both quaternary forms dissociate at the same pH from a mannose 6-phosphate affinity matrix. When starting with dimeric or tetrameric forms, the equilibrium between dimeric and tetrameric forms is reached at pH 7.5 and 4 degrees C after 6-8 days. The presence of 5 mM mannose 6-phosphate shifts the equilibrium toward tetrameric forms. At pH 4.5 and 4 degrees C, the association of dimeric to tetrameric forms is negligible, while tetrameric forms dissociate to dimeric forms within 12 h. The results demonstrate that oligomerization is an intrinsic property of MPR-46 that is affected by ligand binding, pH, and receptor concentration.
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影响因子：
2.9
作者：
J. Schlessinger
通讯作者：
J. Schlessinger
人类非阳离子依赖性甘露糖 6-磷酸受体。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Oshima,A;Nolan,CM;Kyle,JW;Grubb,JH;Sly,WS
通讯作者：
Sly,WS
人转铁蛋白受体的 pH 依赖性可逆构象转变导致自缔合。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Turkewitz,AP;Schwartz,AL;Harrison,SC
通讯作者：
Harrison,SC
DOI：
10.1073/pnas.82.13.4428
发表时间：
1985-01-01
影响因子：
11.1
作者：
HOFLACK, B;KORNFELD, S
通讯作者：
KORNFELD, S
在没有受体寡聚化的情况下表皮生长因子受体酪氨酸蛋白激酶的激活。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Northwood,IC;Davis,RJ
通讯作者：
Davis,RJ