In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C.
In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C.
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DOI：
10.1186/s40104-020-00530-6
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发表时间：
2021-01-11
影响因子：
7
通讯作者：
Waberski D
中科院分区：
文献类型：
--
作者：
Jäkel H;Scheinpflug K;Mühldorfer K;Gianluppi R;Lucca MS;Mellagi APG;Bortolozzo FP;Waberski D
猪精液的低温保存被认为是一种潜在的方法，可以省去授精剂量中的抗生素，从而为全球抗生素耐药性防御战略做出贡献。主要的挑战是精子的冷冻性损伤和精液储存过程中细菌的生长，从而导致生育力下降。研究猪精液在不含抗生素的5 °C低温保存条件下的冷害程度、细菌数量和种类，并评价野外条件下低温保存精液的适用性。将猪射精液加入androstar®Premium，分别在17 °C和5 °C下添加抗生素保存，并用流式细胞仪检测精子功能参数。对生精液和精液标本进行细菌学检查。在一项田间研究中，对194头母猪进行了每日一次授精后的受精率评估。通过活力和膜完整性评估的致命性精子损伤在两个实验组的整个储存过程中都很低。与17 °C相比，在5 °C保存至72 h时，膜流动性低的精子的亚致死性冷冻效果较高(P <； 0.05)，但在144 h后差异无统计学意义。72 h后，在获能液中孵育60 m in的两组精子线粒体膜电位较高，细胞内钙离子含量较低，两组精子的降温效果相似。在5 °C保存的精液中，细菌总数低于103cfu/mL，细菌谱与生精液相似。在34头公猪中，88%的猪冷冻精液符合授精要求。母猪受精率较高，5 °C不含抗生素精液与17 °C含抗生素精液受精母猪之间差异不显著(P >； 0.05)。尽管有微妙的降温作用和低细菌数量，但不含抗生素的猪精液低温储存提供了在猪受精过程中减少抗生素使用的可能性。然而，必须保持严格的卫生指导方针，并认为在现场条件下进一步证明有效是可取的。网上版载有补充材料，可在10.1186/s40104.020-00530-6查阅。
Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main challenges are chilling injury to spermatozoa and bacterial growth during semen storage leading to reduced fertility. To examine chilling injury and the number and type of bacteria in boar semen stored at 5 °C in the absence of antibiotics, and to assess the applicability of hypothermic semen storage under field conditions. Boar ejaculates were extended with AndroStar® Premium, stored at 17 °C with and at 5 °C without antibiotics and tested for functional sperm parameters by flow cytometry. Raw semen and extended samples were investigated bacteriologically. Fertility was evaluated after once-daily inseminations of 194 sows in a field study. Lethal sperm damage assessed by motility and membrane integrity was low throughout storage in both experimental groups. Sublethal chilling effects based on the decrease of viable spermatozoa with low membrane fluidity were higher (P < 0.05) up until 72 h in sperm stored at 5 °C compared to 17 °C but did not differ after 144 h. After 72 h, incubation in capacitating medium for 60 min induced a similar decrease in viable sperm with high mitochondria membrane potential and low cytosolic calcium in both groups. In semen stored at 5 °C, bacteria counts were below 103 CFU/mL and the bacteria spectrum was similar to that of raw semen. In 88% of 34 boars, cooled semen fulfilled the requirements for insemination. Fertility was high and did not differ (P > 0.05) between sow groups inseminated with semen stored antibiotic-free at 5 °C and semen stored at 17 °C with antibiotics. Despite subtle chilling effects and low bacterial numbers, antibiotic-free hypothermic storage of boar semen offers the possibility to reduce the use of antibiotics in pig insemination. However, strict sanitary guidelines must be maintained and further evidence of efficiency under field conditions is considered desirable. The online version contains supplementary material available at 10.1186/s40104-020-00530-6.
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影响因子：
3.7
作者：
Paschoal, Aline F. L.;Luther, Anne-Marie;Waberski, Dagmar
通讯作者：
Waberski, Dagmar
影响因子：
2.2
作者：
Prieto-Martinez, Noelia;Bussalleu, Eva;Yeste, Marc
通讯作者：
Yeste, Marc
影响因子：
2.7
作者：
Casas, I.;Althouse, G. C.
通讯作者：
Althouse, G. C.
影响因子：
2.8
作者：
Menezes, Tila de Alcantara;Goncalves Mellagi, Ana Paula;Bortolozzo, Fernando Pandolfo
通讯作者：
Bortolozzo, Fernando Pandolfo
影响因子：
3.5
作者：
Bucher, K.;Malama, E.;Bollwein, H.
通讯作者：
Bollwein, H.