Regulation of outer membrane protein synthesis in Escherichia coli K-12: deletion of ompC affects expression of the OmpF protein
Regulation of outer membrane protein synthesis in Escherichia coli K-12: deletion of ompC affects expression of the OmpF protein
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大肠杆菌 K-12 外膜蛋白合成的调控：ompC 的缺失影响 OmpF 蛋白的表达
DOI：
10.1128/jb.159.2.555-563.1984
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发表时间：
1984
影响因子：
3.2
通讯作者：
G. McDonald
中科院分区：
文献类型：
--
作者：
C. Schnaitman;G. McDonald
从ompC结构基因上游约8千碱基的Tn10开始的染色体缺失，延伸到携带ompC的2.6千碱基HindIII片段。将2.6千碱基的ompC片段克隆到lambda 540中，得到噬菌体lambda 540C1。当缺失突变体用lambda 540C1溶原时，得到的菌株产生正常水平的OmpC蛋白，并且该蛋白的表达受到渗透、碳源和噬菌体PA-2的lc基因的调控，这表明克隆片段包含了OmpC调控表达所需的所有信息。携带缺失的菌株部分构成了OmpF蛋白的表达，而该菌株和其他ompC突变菌株的lambda 540C1溶原在导致ompC蛋白高水平表达的条件下抑制了OmpF的合成。二倍体或三倍体菌株对OmpF蛋白的合成有较强的抑制作用。我们得出结论，位于ompC编码序列上游的调控元件在有利于ompC表达的条件下抑制OmpF蛋白的翻译。由于已知ompF会抑制ompC的转录，我们认为这两个基因构成了一个封闭的调控环，其作用是放大控制这些蛋白表达的调控信号。
A chromosomal deletion beginning at a Tn10 located ca. 8 kilobases upstream from the ompC structural gene and extending through the 2.6-kilobase HindIII fragment carrying the ompC was isolated. The 2.6-kilobase ompC fragment was cloned into lambda 540 to obtain phage lambda 540C1. When the deletion mutant was lysogenized with lambda 540C1, the resulting strain produced normal levels of OmpC protein, and expression of this protein was regulated by osmolarity, carbon source, and the lc gene of phage PA-2, indicating that the cloned fragment contained all of the information required for regulated expression of ompC. The strain carrying the deletion was partially constitutive for expression of OmpF protein, whereas the lambda 540C1 lysogen of this strain and other strains with mutations in ompC repressed OmpF synthesis under conditions which lead to high-level expression of OmpC protein. Strains which are diploid or triploid for ompC show strong inhibition of synthesis of OmpF protein. We conclude that a regulatory element located upstream from the ompC coding sequence inhibits translation of OmpF protein under conditions which favor OmpC expression. Since ompF is known to repress transcription of ompC, we propose that these two genes constitute a closed regulatory loop which acts to amplify regulatory signals which control expression of these proteins.
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Mizuno,T;Wurtzel,ET;Inouye,M
通讯作者：
Inouye,M
DOI：
10.1073/pnas.81.7.1966
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MIZUNO, T;CHOU, MY;INOUYE, M
通讯作者：
INOUYE, M