Compensatory redistribution of neuroligins and N-cadherin following deletion of synaptic β1-integrin.
Compensatory redistribution of neuroligins and N-cadherin following deletion of synaptic β1-integrin.
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DOI：
10.1002/cne.23027
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发表时间：
2012-06-15
影响因子：
2.5
通讯作者：
Benson, Deanna L.
中科院分区：
文献类型：
--
作者：
Mortillo, Steven;Elste, Alice;Ge, Yongchao;Patil, Shekhar B.;Hsiao, Kuangfu;Huntley, George W.;Davis, Ronald L.;Benson, Deanna L.
β1-containing integrins are required for persistent synaptic potentiation in hippocampus and regulate hippocampal-dependent learning. Based largely on indirect evidence, there is a prevailing assumption that β1-integrins are localized at synapses, where they contribute to synapse adhesion and signaling, but this has not been examined directly. Here, we investigate the fine localization of β1-integrin in adult mouse hippocampus using high-resolution immunogold labeling, with a particular emphasis on synaptic labeling patterns. We find that β1-integrins localize to synapses in CA1 and are concentrated postsynaptically. At the postsynaptic membrane, β1-integrins are found more commonly clustered near active zone centers rather than at the peripheral edges. In mice harboring a conditional deletion of β1-integrins, labeling for N-cadherin and Neuroligins increases. Western blots show increased levels of N-cadherin in total lysates and Neuroligins increase selectively in synaptosomes. These data suggest there is a dynamic, compensatory adjustment of synaptic adhesion. Such adjustment is specific only for certain cell adhesion molecules (CAMs), because labeling for SynCAM is unchanged. Together our findings demonstrate unequivocally that β1-integrin is an integral synaptic adhesion protein, and suggest that adhesive function at the synapse reflects a cooperative and dynamic network of multiple CAM families.
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影响因子：
7.3
作者：
Humphries JD;Byron A;Bass MD;Craig SE;Pinney JW;Knight D;Humphries MJ
通讯作者：
Humphries MJ
影响因子：
3.3
作者：
Chun, D;Gall, CM;Lynch, G
通讯作者：
Lynch, G
影响因子：
4.8
作者：
Conant, K;St Hillaire, C;Nath, A
通讯作者：
Nath, A
影响因子：
5.3
作者：
Chan, CS;Weeber, EJ;Davis, RL
通讯作者：
Davis, RL
影响因子：
2
作者：
Chan, Chi-Shing;Levenson, Jonathan M.;Davis, Ronald L.
通讯作者：
Davis, Ronald L.