Effects of glucose, insulin and triiodothyroxine on leptin and leptin receptor expression and the effects of leptin on activities of enzymes related to glucose metabolism in grass carp (Ctenopharyngodon idella) hepatocytes
Effects of glucose, insulin and triiodothyroxine on leptin and leptin receptor expression and the effects of leptin on activities of enzymes related to glucose metabolism in grass carp (Ctenopharyngodon idella) hepatocytes
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葡萄糖、胰岛素和三碘甲状腺素对草鱼肝细胞瘦素及瘦素受体表达及糖代谢相关酶瘦素活性的影响
DOI：
10.1007/s10695-015-0063-8
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发表时间：
2015-05
影响因子：
2.9
通讯作者：
Zhao, Yu-Hua
中科院分区：
文献类型：
--
作者：
Fang, Liu;Bai, Xiao-Li;Wang, Min;Zhao, Yu-Hua
瘦素是哺乳动物食欲和能量消耗的重要调节因子，但其在鱼类代谢控制中的作用尚不清楚。我们之前的研究表明，瘦素对草鱼（tenopharyngodon idella）的食物摄入和能量消耗以及脂质代谢（刺激脂肪分解和抑制脂肪生成）有调节作用。为了进一步研究瘦素在鱼类中的作用，本研究探讨了葡萄糖、胰岛素和三碘甲状腺素（T3）对草鱼肝细胞中瘦素和瘦素受体（Lepr）表达水平的影响，以及瘦素对草鱼肝细胞中关键糖代谢酶活性的影响。我们的数据表明，葡萄糖诱导瘦素基因表达呈剂量依赖性，而Lepr基因表达呈双相变化。高剂量胰岛素（100 ng/mL）显著上调瘦素和Lepr的表达。低浓度T3显著上调瘦素表达，高浓度T3抑制瘦素表达。T3以剂量依赖的方式上调Lepr的表达。综上所述，瘦素与葡萄糖、胰岛素和T3三个因子关系密切，可能参与草鱼糖代谢的调节。此外，我们还发现瘦素影响了参与葡萄糖代谢的关键酶的活性，这可能是通过胰岛素受体底物-磷酸肌醇3激酶信号传导介导的。
Leptin is an important regulator of appetite and energy expenditure in mammals, but its role in fish metabolism control is poorly understood. Our previous studies demonstrated that leptin has an effect on the regulation of food intake and energy expenditure as well as lipid metabolism (stimulation of lipolysis and inhibition of adipogenesis) in the grass carpCtenopharyngodon idella. To further investigate the role of leptin in fish, the effects of glucose, insulin and triiodothyroxine (T3) on the expression levels of leptin and leptin receptor (Lepr) and the effects of leptin on the activities of critical glucose metabolism enzymes in grass carp hepatocytes were evaluated in the present study. Our data indicated that leptin gene expression was induced by glucose in a dose-dependent manner, while Lepr gene expression exhibited a biphasic change. A high dose of insulin (100 ng/mL) significantly up-regulated the expression of leptin and Lepr. Leptin expression was markedly up-regulated by a low concentration of T3 but inhibited by a high concentration of T3. T3 up-regulated Lepr expression in a dose-dependent manner. Together, these data suggest that leptin had a close relationship with three factors (glucose, insulin and T3) and might participate in the regulation of glucose metabolism in grass carp. In addition, we also found that leptin affected the activities of key enzymes that are involved in glucose metabolism, which might be mediated by insulin receptor substrate-phosphoinositol 3-kinase signaling.
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影响因子：
5.8
作者：
Swoap, Steven J.
通讯作者：
Swoap, Steven J.
影响因子：
2.7
作者：
Li, Guan-Gui;Liang, Xu-Fang;Lai, Kaaseng
通讯作者：
Lai, Kaaseng
DOI：
10.1016/j.bbalip.2004.01.007
发表时间：
2004-06
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
G. Medina-Gómez;R. Calvo;M. Obregon
通讯作者：
G. Medina-Gómez;R. Calvo;M. Obregon
影响因子：
10
作者：
A. Hulbert
通讯作者：
A. Hulbert
影响因子：
7.7
作者：
Denroche HC;Levi J;Wideman RD;Sequeira RM;Huynh FK;Covey SD;Kieffer TJ
通讯作者：
Kieffer TJ