The G1 phase optical reporter serves as a sensor of CDK4/6 inhibition in vivo.
The G1 phase optical reporter serves as a sensor of CDK4/6 inhibition in vivo.
复制标题
G1 期光学报告基因充当体内 CDK4/6 抑制的传感器。
DOI：
10.7150/ijbs.52101
复制
发表时间：
2021
影响因子：
9.2
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Guo C;Guo Y;Liu J;Gao Y;Wei M;Zhao R;Chen M;Zhang G
Visualization of cell-cycle G1 phase for monitoring the early response of cell cycle specific drug remains challenging. In this study, we developed genetically engineered bioluminescent reporters by fusing full-length cyclin E to the C-terminal luciferase (named as CycE-Luc and CycE-Luc2). Next, HeLa cell line or an ER-positive breast cancer cell line MCF-7 was transfected with these reporters. In cellular assays, the bioluminescent signal of CycE-Luc and CycE-Luc2 was accumulated in the G1 phase and decreased after exiting from the G1 phase. The expression of CycE-Luc and CycE-Luc2 fusion protein was regulated in a cell cycle-dependent manner, which was mediated by proteasome ubiquitination and degradation. Next, our in vitro and in vivo experiment confirmed that the cell cycle arrested by anti-cancer agents (palbociclib or 5-FU) was monitored quantitatively and dynamically by bioluminescent imaging of these reporters in a real-time and non-invasive manner. Thus, these optical reporters could reflect the G1 phase alternation of cell cycle, and might become a future clinically translatable approach for predicting and monitoring response to palbociclib in patients with ER-positive breast cancer.
登录
查看更多内容
影响因子：
8.4
作者：
Eisenhauer, E. A.;Therasse, P.;Verweij, J.
通讯作者：
Verweij, J.
活体动物细胞周期 DNA 合成阶段的生物发光成像
影响因子：
3.7
作者：
Chen ZH;Zhao RJ;Li RH;Guo CP;Zhang GJ
通讯作者：
Zhang GJ
影响因子：
8
作者：
Dean, J. L.;Thangavel, C.;Knudsen, E. S.
通讯作者：
Knudsen, E. S.
影响因子：
64.5
作者：
Sakaue-Sawano, Asako;Kurokawa, Hiroshi;Miyawaki, Atsushi
通讯作者：
Miyawaki, Atsushi
影响因子：
4.3
作者：
Averbeck, Nicole B.;Ringel, Oliver;Taucher-Scholz, Gisela
通讯作者：
Taucher-Scholz, Gisela