Prognostic and Immune Implications of a Novel Pyroptosis-Related Five-Gene Signature in Breast Cancer.
Prognostic and Immune Implications of a Novel Pyroptosis-Related Five-Gene Signature in Breast Cancer.
复制标题
乳腺癌中新型焦亡相关五基因特征的预后和免疫影响
DOI：
10.3389/fsurg.2022.837848
复制
发表时间：
2022
影响因子：
1.8
通讯作者：
Li, Man
中科院分区：
文献类型：
--
作者：
Zheng, Yuanyuan;Wang, Kainan;Li, Ning;Zhang, Qianran;Chen, Fengxi;Li, Man
乳腺癌（BC）是全世界女性中最常见的癌症，具有巨大的异质性。焦亡对肿瘤的发生和发展有重要影响。尽管如此，脓毒性相关基因（PRG）和BC免疫微环境之间可能的相关性还有待研究。在癌症基因组图谱乳腺癌队列中，38个PRG在恶性和非恶性乳腺组织之间显示出显著差异。38个PRG的一致聚类将1，089个个体分为两种与热解相关（PR）的模式。使用单变量和LASSO-Cox分析，基于两个聚类之间的差异表达基因构建PR五基因预测特征。使用表达数据（ESTIMATE）评估恶性肿瘤中的基质和免疫细胞，通过估计RNA转录物的相对子集（CIBERSORT）和单样本基因集富集分析（ssGSEA）进行细胞类型鉴定的工具用于研究BC肿瘤微环境（TME）。在TME中，两个PR簇显示出不同的临床病理特征、生存结局和免疫细胞浸润特征。开发的五个特征模型（SEMA 3B，IGKC，KLRB 1，BIRC 3和PSME 2）根据估计的中位风险评分将BC患者分为两个风险组。低评分组的患者生存率较高，免疫细胞浸润范围更广。外部验证集可以产生类似的结果。我们的数据表明，PRG对BC免疫微环境有显着影响。PR簇和相关的预测特征刺激了对焦亡的额外研究，以优化BC患者的治疗策略及其对免疫治疗的反应。
Breast cancer (BC) is the most common cancer among women worldwide, with enormous heterogeneity. Pyroptosis has a significant impact on the development and progression of tumors. Nonetheless, the possible correlation between pyroptosis-related genes (PRGs) and the BC immune microenvironment has yet to be investigated. In The Cancer Genome Atlas Breast Cancer cohort, 38 PRGs were shown to be significantly different between malignant and non-malignant breast tissues. The 38 PRGs’ consensus clustering grouped 1,089 individuals into two pyroptosis-related (PR) patterns. Using univariate and LASSO-Cox analyses, a PR five-gene predictive signature was constructed based on the differentially expressed genes between two clusters. The tools estimation of stromal and immune cells in malignant tumours using expression data (ESTIMATE), cell type identification by estimating relative subsets Of RNA transcripts (CIBERSORT), and single-sample gene set enrichment analysis (ssGSEA) were used to investigate the BC tumor microenvironment (TME). In TME, the two PR clusters displayed distinct clinicopathological characteristics, survival outcomes, and immunocyte infiltration features. The developed five-signature model (SEMA3B, IGKC, KLRB1, BIRC3, and PSME2) classified BC patients into two risk groups based on the estimated median risk score. Patients in the low-scoring category had a higher chance of survival and more extensive immunocyte infiltration. An external validation set can yield similar results. Our data suggest that PRGs have a significant impact on the BC immunological microenvironment. The PR clusters and associated predictive signature stimulate additional research into pyroptosis in order to optimize therapeutic strategies for BC patients and their responses to immune therapy.
登录
查看更多内容
影响因子：
2
作者：
Calkins, Hugh;Hindricks, Gerhard;Yamane, Teiichi
通讯作者：
Yamane, Teiichi
影响因子：
9.7
作者：
Mendoza-Rodriguez, Monica;Arevalo Romero, Haruki;Meza, Isaura
通讯作者：
Meza, Isaura
影响因子：
8.7
作者：
Man SM;Kanneganti TD
通讯作者：
Kanneganti TD
影响因子：
64.8
作者：
Ding, Jingjin;Wang, Kun;Shao, Feng
通讯作者：
Shao, Feng
影响因子：
11.5
作者：
Asslaber, Daniela;Wacht, Nathalie;Egle, Alexander
通讯作者：
Egle, Alexander