Intracellular distribution of 73,000 and 72,000 dalton heat shock proteins in HeLa cells.
Intracellular distribution of 73,000 and 72,000 dalton heat shock proteins in HeLa cells.
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HeLa 细胞中 73,000 和 72,000 道尔顿热休克蛋白的细胞内分布。
DOI：
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发表时间：
1986
影响因子：
3.1
通讯作者：
C. Sato
中科院分区：
文献类型：
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作者：
K. Ohtsuka;H. Nakamura;C. Sato
用间接免疫荧光染色法研究了热休克和化学应激处理的HeLa细胞中73，000和72，000道尔顿热休克蛋白（HSP 73/72）的细胞内定位。所用的抗血清特异性地识别HeLa细胞中的HSP 73/72，并且通过在42摄氏度下加热细胞2或4小时以及通过用化学应激物（亚砷酸钠、氯化镉、8-羟基喹啉和乙醇）预先处理来增加HSP。在37 ℃下有弥漫的细胞质染色，而在42 ℃下加热2小时时，核仁染色明亮。HSP 73/72在核仁中的这种快速积累不受放线菌酮（50微克/毫升）的抑制。热休克蛋白的易位到核仁是特定的热，因为没有易位是由化学应激处理诱导。当细胞加热后恢复到37 ℃时，其核仁中的HSPs迅速消失，6-9 h后出现弥漫性胞质染色。我们的研究结果表明，瞬时积累的HSP 73/72在HeLa细胞的核仁，是由热休克诱导的是不相关的发展，在其他细胞系统中获得的耐热性。
Intracellular localization of 73,000 and 72,000 dalton heat shock proteins (HSP73/72) in HeLa cells that were heat shocked or treated with chemical stressors was investigated using indirect immunofluorescent staining. The antiserum used specifically recognized the HSP73/72 in HeLa cells, and HSPs were increased by heating cells at 42 degrees C for 2 or 4 h and by prior treatment with chemical stressors (sodium arsenite, cadmium chloride, 8-hydroxyquinoline and ethanol). There was diffuse cytoplasmic staining at 37 degrees C, whereas nucleoli were stained brightly when cells were heated at 42 degrees C for 2 h. This rapid accumulation of HSP73/72 in the nucleoli was not inhibited by cycloheximide (50 micrograms/ml). Translocation of HSPs to the nucleoli was specific for heat because no translocation was induced by treatment with chemical stressors. When the cells were returned to 37 degrees C after heating, the HSPs in their nucleoli disappeared rapidly and diffuse cytoplasmic staining was present after 6-9 h. Our results suggest that the transient accumulation of HSP73/72 in HeLa cell nucleoli that is induced by heat shock is not correlated with the development of thermotolerance obtained in other cell systems.
哺乳动物 70,000 道尔顿应激蛋白的快速纯化：蛋白质对核苷酸的亲和力。
影响因子：
5.3
作者：
Welch,WJ;Feramisco,JR
通讯作者：
Feramisco,JR
DOI：
10.1073/pnas.79.10.3218
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
LI, GC;WERB, Z
通讯作者：
WERB, Z