Response of adenylate cyclase to parathyroid hormone and prostaglandins by human isolated glomeruli.
Response of adenylate cyclase to parathyroid hormone and prostaglandins by human isolated glomeruli.
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人分离肾小球腺苷酸环化酶对甲状旁腺激素和前列腺素的反应。
DOI：
10.1210/jcem-57-6-1207
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
R. Ardaillou
中科院分区：
文献类型：
--
作者：
N. Ardaillou;M. Nivez;M. Schambelan;R. Ardaillou
根据大鼠微穿刺研究的结果以及从大鼠和兔子肾皮质分离的肾小球中激素反应性腺苷酸环化酶活性的证明，推断出激素物质和其他生化信使在肾小球滤过率调节中的作用。为了研究这些激素因素是否有助于调节人类肾小球功能，我们研究了从四个人类肾脏的肾皮质中分离出的肾小球给予人 PTH-(1-34) 和前列腺素 (PG) 时腺苷酸环化酶活性的反应。 PTH 和 PG（PGE2、PGI2，以及较小程度的 PGF2α）刺激人肾小球腺苷酸环化酶活性。基础腺苷酸环化酶活性范围为 0.2-1.2 nmol 20 min-1 mg-1。对于每种激动剂，高于基础值的百分比增加（在最大测试浓度下）和引发 50% 最大刺激 (ED50) 的激动剂浓度如下： PTH 300-460% (10,000 mIU/ml)； ED50，100-550 mIU/ml；对于 PGE2，160-380% (10 microM)； ED50，0.4-1.6 微摩尔；对于 PGI2，180-650% (10 microM)； ED50，0.09-0.46 微摩尔。合成的鸟苷酸 5'-鸟苷酰亚胺二磷酸 [Gpp(NH)p] 增强了 PTH 和 PGI2 的作用，因为组合作用大于单个激动剂作用的总和，并且三因素方差分析表明，Gpp(NH)p 与 PTH 或 PGI2 之间存在显着的相互作用。观察到 PGE2 具有加和性，但没有增强作用。 PTH 与 PGE2 或 PGI2 的作用也是相加的，这一发现表明 PTH 和 PG 与同一腺苷酸环化酶池无关。相比之下，PGE2 和 PGI2 的组合产生的效果明显低于其单独效果的总和，这一发现表明这些 PG 部分共享相同的腺苷酸环化酶库。人分离肾小球中 PTH 和 PG 依赖性腺苷酸环化酶活性的证明表明这些激动剂在调节人肾小球滤过率中的作用。
A role for hormonal substances and other biochemical messengers in the regulation of the glomerular filtration rate has been inferred from the results of micropuncture studies in the rat and from the demonstration of hormone-responsive adenylate cyclase activity in glomeruli isolated from the renal cortex of rats and rabbits. To investigate whether such hormonal factors may contribute to the regulation of glomerular function in humans, we studied the response of adenylate cyclase activity to the administration of human PTH-(1-34) and prostaglandins (PG) by glomeruli isolated from the renal cortex of four human kidneys. PTH and PGs (PGE2, PGI2, and, to a lesser extent, PGF2 alpha) stimulated human glomerular adenylate cyclase activity. Basal adenylate cyclase activity ranged from 0.2-1.2 nmol 20 min-1 mg-1. For each agonist, the percent increase above basal values (at the maximum concentration tested) and the concentration of the agonist that elicited 50% of the maximum stimulation (ED50) were as follows: for PTH 300-460% (10,000 mIU/ml); ED50, 100-550 mIU/ml; for PGE2, 160-380% (10 microM); ED50, 0.4-1.6 microM; and for PGI2, 180-650% (10 microM); ED50, 0.09-0.46 microM. The synthetic guanylnucleotide 5'-guanylylimidodiphosphate [Gpp(NH)p] potentiated the effect of PTH and PGI2, since the combined effects were greater than the sums of the effects of the individual agonists, and there was a significant interaction between Gpp(NH)p and PTH or PGI2, as indicated by three-factor analysis of variance. Additivity, but not potentiation, was observed for PGE2. The effects of PTH plus PGE2 or PGI2 were also additive, a finding that suggests that PTH and PGs are not linked to the same pool of adenylate cyclase. In contrast, the combination of PGE2 and PGI2 resulted in a significantly lower effect than the sum of their individual effects, a finding indicating that these PGs share in part a common pool of adenylate cyclase. Demonstration of PTH- and PG-dependent adenylate cyclase activity in human isolated glomeruli suggests a role for these agonists in the regulation of the glomerular filtration rate in man.
远端肾单位功能的激素控制。
DOI：
10.1007/bf01738140
发表时间：
1985
期刊：
Klinische Wochenschrift
影响因子：
--
作者：
Giebisch,G
通讯作者：
Giebisch,G
鸡肾质膜中甲状旁腺激素刺激的腺苷酸环化酶的鸟苷酸增强：不依赖于受体的效应。
影响因子：
4.8
作者：
Nissenson,RA;Nyiredy,KO;Arnaud,CD
通讯作者：
Arnaud,CD